The identification of F actin in the pollen tube and protoplast of Amaryllis belladonna

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The production of protoplasts from the pollen of Amaryllis belladonna has facilitated a more direct investigation of the physiological and mechano-chemical basis of streaming. Cytoplasm is removed from an actively streaming protoplast or intact pollen tube and layered on a coated grid in a solution containing a low free calcium ion concentration. Under these conditions 6 nm thin filaments are observed. The thin filaments are morphologically identical with F actin and bind rabbit muscle HMM, forming characteristic arrowhead complexes that are dissociated by subsequent treatment with MgATP.

Original languageEnglish (US)
Pages (from-to)435-439
Number of pages5
JournalExperimental Cell Research
Volume88
Issue number2
DOIs
StatePublished - 1974
Externally publishedYes

Fingerprint

Atropa belladonna
Pollen Tube
Protoplasts
Actins
Sagittaria
Pollen
Cytoplasm
Adenosine Triphosphate
Ions
Rabbits
Calcium
Muscles

ASJC Scopus subject areas

  • Cell Biology

Cite this

The identification of F actin in the pollen tube and protoplast of Amaryllis belladonna. / Condeelis, John S.

In: Experimental Cell Research, Vol. 88, No. 2, 1974, p. 435-439.

Research output: Contribution to journalArticle

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