Abstract
The production of protoplasts from the pollen of Amaryllis belladonna has facilitated a more direct investigation of the physiological and mechano-chemical basis of streaming. Cytoplasm is removed from an actively streaming protoplast or intact pollen tube and layered on a coated grid in a solution containing a low free calcium ion concentration. Under these conditions 6 nm thin filaments are observed. The thin filaments are morphologically identical with F actin and bind rabbit muscle HMM, forming characteristic arrowhead complexes that are dissociated by subsequent treatment with MgATP.
Original language | English (US) |
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Pages (from-to) | 435-439 |
Number of pages | 5 |
Journal | Experimental Cell Research |
Volume | 88 |
Issue number | 2 |
DOIs | |
State | Published - Oct 1974 |
Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology