The identification and properties of chitin-binding protein b01 (CBPb01)

Masako Suzuki, Masami Morimatsu, Bunei Syuto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bovine serum contains N-acetyl-D-glucosamine (GlcNAc)-sensitive opsonin inhibitory factors. In the present study, a major component of chitin-binding protein (chitin-binding protein b01, CBPb01) was purified from bovine serum, and identified CBPb01 as bovine IgM by its subunit structure, antigenic properties, and partial sequences. The results of a lectin-binding assay showed that the heavy chain of CBPb01 had a GlcNAc structure, but the commercial IgM did not. It is possible that CBPb01 interconnects through its GlcNAc structure, subsequently forming complexes. We also demonstrated that CBPb01 had opsonin-inhibitory activity, and that this activity was dependent on the binding of CBPb01 to GlcNAc on the zymosan surface. These findings indicate the presence of a kind of IgM that recognizes GlcNAc structure in the regulation of opsonization.

Original languageEnglish (US)
Pages (from-to)477-481
Number of pages5
JournalJournal of Veterinary Medical Science
Volume64
Issue number6
DOIs
StatePublished - Jun 2002
Externally publishedYes

Fingerprint

Chitin
chitin
binding proteins
Carrier Proteins
opsonins
Opsonin Proteins
Immunoglobulin M
cattle
zymosan
Zymosan
Acetylglucosamine
glucosamine
Protein
Serum
Lectins
Protein Binding
lectins
assays
Bovine

Keywords

  • Bovine serum
  • Chitin
  • GlcNAc
  • IgM
  • Opsonin-regulatory protein

ASJC Scopus subject areas

  • veterinary(all)
  • Marketing

Cite this

The identification and properties of chitin-binding protein b01 (CBPb01). / Suzuki, Masako; Morimatsu, Masami; Syuto, Bunei.

In: Journal of Veterinary Medical Science, Vol. 64, No. 6, 06.2002, p. 477-481.

Research output: Contribution to journalArticle

Suzuki, Masako ; Morimatsu, Masami ; Syuto, Bunei. / The identification and properties of chitin-binding protein b01 (CBPb01). In: Journal of Veterinary Medical Science. 2002 ; Vol. 64, No. 6. pp. 477-481.
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