The homeostasis of iron, copper, and zinc in Paracoccidioides brasiliensis, Cryptococcus neoformans var. Grubii, and Cryptococcus gattii: A comparative analysis

Mirelle Garcia Silva, Augusto Schrank, Elisa Flávia L C Bailão, Alexandre Melo Bailão, Clayton Luiz Borges, Charley Christian Staats, Juliana Alves Parente, Maristela Pereira, Silvia Maria Salem-Izacc, Maria José Soares Mendes-Giannini, Rosely Maria Zancopé Oliveira, Lívia Kmetzsch Rosa e Silva, Joshua D. Nosanchuk, Marilene Henning Vainstein, Célia Maria de Almeida Soares

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Iron, copper, and zinc are essential for all living organisms. Moreover, the homeostasis of these metals is vital to microorganisms during pathogenic interactions with a host. Most pathogens have developed specific mechanisms for the uptake of micronutrients from their hosts in order to counteract the low availability of essential ions in infected tissues. We report here an analysis of genes potentially involved in iron, copper, and zinc uptake and homeostasis in the fungal pathogens Paracoccidioides brasiliensis, Cryptococcus neoformans var. grubii, and Cryptococcus gattii. Although prior studies have identified certain aspects of metal regulation in Cryptococcus species, little is known regarding the regulation of these elements in P. brasiliensis. We also present amino acid sequences analyses of deduced proteins in order to examine possible conserved domains. The genomic data reveals, for the first time, genes associated to iron, copper, and zinc assimilation and homeostasis in P. brasiliensis. Furthermore, analyses of the three fungal species identified homologs to genes associated with high-affinity uptake systems, vacuolar and mitochondrial iron storage, copper uptake and reduction, and zinc assimilation. However, homologs to genes involved in siderophore production were only found in P. brasiliensis. Interestingly, in silico analysis of the genomes of P. brasiliensis Pb01, Pb03, and Pb18 revealed significant differences in the presence and/or number of genes involved in metal homeostasis, such as in genes related to iron reduction and oxidation. The broad analyses of the genomes of P. brasiliensis, C. neoformans var. grubii, and C. gattii for genes involved in metal homeostasis provide important groundwork for numerous interesting future areas of investigation that are required in order to validate and explore the function of the identified genes and gene pathways.

Original languageEnglish (US)
Article numberArticle 49
JournalFrontiers in Microbiology
Volume2
Issue numberMAR
DOIs
StatePublished - 2011

Fingerprint

Cryptococcus gattii
Paracoccidioides
Cryptococcus neoformans
Zinc
Copper
Homeostasis
Iron
Genes
Metals
Genome
Cryptococcus
Siderophores
Micronutrients
Protein Sequence Analysis
Computer Simulation

Keywords

  • Infection
  • Micronutrient homeostasis
  • Pathogenic fungi

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Silva, M. G., Schrank, A., Bailão, E. F. L. C., Bailão, A. M., Borges, C. L., Staats, C. C., ... Soares, C. M. D. A. (2011). The homeostasis of iron, copper, and zinc in Paracoccidioides brasiliensis, Cryptococcus neoformans var. Grubii, and Cryptococcus gattii: A comparative analysis. Frontiers in Microbiology, 2(MAR), [Article 49]. https://doi.org/10.3389/fmicb.2011.00049

The homeostasis of iron, copper, and zinc in Paracoccidioides brasiliensis, Cryptococcus neoformans var. Grubii, and Cryptococcus gattii : A comparative analysis. / Silva, Mirelle Garcia; Schrank, Augusto; Bailão, Elisa Flávia L C; Bailão, Alexandre Melo; Borges, Clayton Luiz; Staats, Charley Christian; Parente, Juliana Alves; Pereira, Maristela; Salem-Izacc, Silvia Maria; Mendes-Giannini, Maria José Soares; Oliveira, Rosely Maria Zancopé; Silva, Lívia Kmetzsch Rosa e; Nosanchuk, Joshua D.; Vainstein, Marilene Henning; Soares, Célia Maria de Almeida.

In: Frontiers in Microbiology, Vol. 2, No. MAR, Article 49, 2011.

Research output: Contribution to journalArticle

Silva, MG, Schrank, A, Bailão, EFLC, Bailão, AM, Borges, CL, Staats, CC, Parente, JA, Pereira, M, Salem-Izacc, SM, Mendes-Giannini, MJS, Oliveira, RMZ, Silva, LKRE, Nosanchuk, JD, Vainstein, MH & Soares, CMDA 2011, 'The homeostasis of iron, copper, and zinc in Paracoccidioides brasiliensis, Cryptococcus neoformans var. Grubii, and Cryptococcus gattii: A comparative analysis', Frontiers in Microbiology, vol. 2, no. MAR, Article 49. https://doi.org/10.3389/fmicb.2011.00049
Silva, Mirelle Garcia ; Schrank, Augusto ; Bailão, Elisa Flávia L C ; Bailão, Alexandre Melo ; Borges, Clayton Luiz ; Staats, Charley Christian ; Parente, Juliana Alves ; Pereira, Maristela ; Salem-Izacc, Silvia Maria ; Mendes-Giannini, Maria José Soares ; Oliveira, Rosely Maria Zancopé ; Silva, Lívia Kmetzsch Rosa e ; Nosanchuk, Joshua D. ; Vainstein, Marilene Henning ; Soares, Célia Maria de Almeida. / The homeostasis of iron, copper, and zinc in Paracoccidioides brasiliensis, Cryptococcus neoformans var. Grubii, and Cryptococcus gattii : A comparative analysis. In: Frontiers in Microbiology. 2011 ; Vol. 2, No. MAR.
@article{ec471fd02ad441559a5247a958644930,
title = "The homeostasis of iron, copper, and zinc in Paracoccidioides brasiliensis, Cryptococcus neoformans var. Grubii, and Cryptococcus gattii: A comparative analysis",
abstract = "Iron, copper, and zinc are essential for all living organisms. Moreover, the homeostasis of these metals is vital to microorganisms during pathogenic interactions with a host. Most pathogens have developed specific mechanisms for the uptake of micronutrients from their hosts in order to counteract the low availability of essential ions in infected tissues. We report here an analysis of genes potentially involved in iron, copper, and zinc uptake and homeostasis in the fungal pathogens Paracoccidioides brasiliensis, Cryptococcus neoformans var. grubii, and Cryptococcus gattii. Although prior studies have identified certain aspects of metal regulation in Cryptococcus species, little is known regarding the regulation of these elements in P. brasiliensis. We also present amino acid sequences analyses of deduced proteins in order to examine possible conserved domains. The genomic data reveals, for the first time, genes associated to iron, copper, and zinc assimilation and homeostasis in P. brasiliensis. Furthermore, analyses of the three fungal species identified homologs to genes associated with high-affinity uptake systems, vacuolar and mitochondrial iron storage, copper uptake and reduction, and zinc assimilation. However, homologs to genes involved in siderophore production were only found in P. brasiliensis. Interestingly, in silico analysis of the genomes of P. brasiliensis Pb01, Pb03, and Pb18 revealed significant differences in the presence and/or number of genes involved in metal homeostasis, such as in genes related to iron reduction and oxidation. The broad analyses of the genomes of P. brasiliensis, C. neoformans var. grubii, and C. gattii for genes involved in metal homeostasis provide important groundwork for numerous interesting future areas of investigation that are required in order to validate and explore the function of the identified genes and gene pathways.",
keywords = "Infection, Micronutrient homeostasis, Pathogenic fungi",
author = "Silva, {Mirelle Garcia} and Augusto Schrank and Bail{\~a}o, {Elisa Fl{\'a}via L C} and Bail{\~a}o, {Alexandre Melo} and Borges, {Clayton Luiz} and Staats, {Charley Christian} and Parente, {Juliana Alves} and Maristela Pereira and Salem-Izacc, {Silvia Maria} and Mendes-Giannini, {Maria Jos{\'e} Soares} and Oliveira, {Rosely Maria Zancop{\'e}} and Silva, {L{\'i}via Kmetzsch Rosa e} and Nosanchuk, {Joshua D.} and Vainstein, {Marilene Henning} and Soares, {C{\'e}lia Maria de Almeida}",
year = "2011",
doi = "10.3389/fmicb.2011.00049",
language = "English (US)",
volume = "2",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
number = "MAR",

}

TY - JOUR

T1 - The homeostasis of iron, copper, and zinc in Paracoccidioides brasiliensis, Cryptococcus neoformans var. Grubii, and Cryptococcus gattii

T2 - A comparative analysis

AU - Silva, Mirelle Garcia

AU - Schrank, Augusto

AU - Bailão, Elisa Flávia L C

AU - Bailão, Alexandre Melo

AU - Borges, Clayton Luiz

AU - Staats, Charley Christian

AU - Parente, Juliana Alves

AU - Pereira, Maristela

AU - Salem-Izacc, Silvia Maria

AU - Mendes-Giannini, Maria José Soares

AU - Oliveira, Rosely Maria Zancopé

AU - Silva, Lívia Kmetzsch Rosa e

AU - Nosanchuk, Joshua D.

AU - Vainstein, Marilene Henning

AU - Soares, Célia Maria de Almeida

PY - 2011

Y1 - 2011

N2 - Iron, copper, and zinc are essential for all living organisms. Moreover, the homeostasis of these metals is vital to microorganisms during pathogenic interactions with a host. Most pathogens have developed specific mechanisms for the uptake of micronutrients from their hosts in order to counteract the low availability of essential ions in infected tissues. We report here an analysis of genes potentially involved in iron, copper, and zinc uptake and homeostasis in the fungal pathogens Paracoccidioides brasiliensis, Cryptococcus neoformans var. grubii, and Cryptococcus gattii. Although prior studies have identified certain aspects of metal regulation in Cryptococcus species, little is known regarding the regulation of these elements in P. brasiliensis. We also present amino acid sequences analyses of deduced proteins in order to examine possible conserved domains. The genomic data reveals, for the first time, genes associated to iron, copper, and zinc assimilation and homeostasis in P. brasiliensis. Furthermore, analyses of the three fungal species identified homologs to genes associated with high-affinity uptake systems, vacuolar and mitochondrial iron storage, copper uptake and reduction, and zinc assimilation. However, homologs to genes involved in siderophore production were only found in P. brasiliensis. Interestingly, in silico analysis of the genomes of P. brasiliensis Pb01, Pb03, and Pb18 revealed significant differences in the presence and/or number of genes involved in metal homeostasis, such as in genes related to iron reduction and oxidation. The broad analyses of the genomes of P. brasiliensis, C. neoformans var. grubii, and C. gattii for genes involved in metal homeostasis provide important groundwork for numerous interesting future areas of investigation that are required in order to validate and explore the function of the identified genes and gene pathways.

AB - Iron, copper, and zinc are essential for all living organisms. Moreover, the homeostasis of these metals is vital to microorganisms during pathogenic interactions with a host. Most pathogens have developed specific mechanisms for the uptake of micronutrients from their hosts in order to counteract the low availability of essential ions in infected tissues. We report here an analysis of genes potentially involved in iron, copper, and zinc uptake and homeostasis in the fungal pathogens Paracoccidioides brasiliensis, Cryptococcus neoformans var. grubii, and Cryptococcus gattii. Although prior studies have identified certain aspects of metal regulation in Cryptococcus species, little is known regarding the regulation of these elements in P. brasiliensis. We also present amino acid sequences analyses of deduced proteins in order to examine possible conserved domains. The genomic data reveals, for the first time, genes associated to iron, copper, and zinc assimilation and homeostasis in P. brasiliensis. Furthermore, analyses of the three fungal species identified homologs to genes associated with high-affinity uptake systems, vacuolar and mitochondrial iron storage, copper uptake and reduction, and zinc assimilation. However, homologs to genes involved in siderophore production were only found in P. brasiliensis. Interestingly, in silico analysis of the genomes of P. brasiliensis Pb01, Pb03, and Pb18 revealed significant differences in the presence and/or number of genes involved in metal homeostasis, such as in genes related to iron reduction and oxidation. The broad analyses of the genomes of P. brasiliensis, C. neoformans var. grubii, and C. gattii for genes involved in metal homeostasis provide important groundwork for numerous interesting future areas of investigation that are required in order to validate and explore the function of the identified genes and gene pathways.

KW - Infection

KW - Micronutrient homeostasis

KW - Pathogenic fungi

UR - http://www.scopus.com/inward/record.url?scp=79960858391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960858391&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2011.00049

DO - 10.3389/fmicb.2011.00049

M3 - Article

C2 - 21833306

AN - SCOPUS:79960858391

VL - 2

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - MAR

M1 - Article 49

ER -