The histone variant MacroH2A1 regulates target gene expression in part by recruiting the transcriptional coregulator PELP1

Kristine M. Hussey, Hongshan Chen, Christine Yang, Eugene Park, Nasun Hah, Hediye Erdjument-Bromage, Paul Tempst, Matthew J. Gamble, W. Lee Kraus

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

MacroH2A1 is a histone variant harboring an ~25-kDa carboxyl-terminal macrodomain. Due to its enrichment on the inactive X chromosome, macroH2A1 was thought to play a role in transcriptional repression. However, recent studies have shown that macroH2A1 occupies autosomal chromatin and regulates genes in a context-specific manner. The macrodomain may play a role in the modulation of gene expression outcomes via physical interactions with effector proteins, which may depend on the ability of the macrodomain to bind NAD+ metabolite ligands. Here, we identify proline, glutamic acid, and leucine-rich protein 1 (PELP1), a chromatin-associated factor and transcriptional coregulator, as a ligand-independent macrodomain-interacting factor. We used chromatin immunoprecipitation coupled with tiling microarrays (ChIP-chip) to determine the genomic localization of PELP1 in MCF-7 human breast cancer cells. We find that PELP1 genomic localization is highly correlated with that of macroH2A1. Additionally, PELP1 positively correlates with heterochromatic chromatin marks and negatively correlates with active transcription marks, much like macroH2A1. MacroH2A1 specifically recruits PELP1 to the promoters of macroH2A1 target genes, but macroH2A1 occupancy occurs independent of PELP1. This recruitment allows macroH2A1 and PELP1 to cooperatively regulate gene expression outcomes.

Original languageEnglish (US)
Pages (from-to)2437-2449
Number of pages13
JournalMolecular and Cellular Biology
Volume34
Issue number13
DOIs
StatePublished - 2014

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Histones
Gene Expression
Chromatin
Ligands
Chromatin Immunoprecipitation
X Chromosome
human PELP1 protein
NAD
Genes
Breast Neoplasms
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The histone variant MacroH2A1 regulates target gene expression in part by recruiting the transcriptional coregulator PELP1. / Hussey, Kristine M.; Chen, Hongshan; Yang, Christine; Park, Eugene; Hah, Nasun; Erdjument-Bromage, Hediye; Tempst, Paul; Gamble, Matthew J.; Kraus, W. Lee.

In: Molecular and Cellular Biology, Vol. 34, No. 13, 2014, p. 2437-2449.

Research output: Contribution to journalArticle

Hussey, KM, Chen, H, Yang, C, Park, E, Hah, N, Erdjument-Bromage, H, Tempst, P, Gamble, MJ & Kraus, WL 2014, 'The histone variant MacroH2A1 regulates target gene expression in part by recruiting the transcriptional coregulator PELP1', Molecular and Cellular Biology, vol. 34, no. 13, pp. 2437-2449. https://doi.org/10.1128/MCB.01315-13
Hussey, Kristine M. ; Chen, Hongshan ; Yang, Christine ; Park, Eugene ; Hah, Nasun ; Erdjument-Bromage, Hediye ; Tempst, Paul ; Gamble, Matthew J. ; Kraus, W. Lee. / The histone variant MacroH2A1 regulates target gene expression in part by recruiting the transcriptional coregulator PELP1. In: Molecular and Cellular Biology. 2014 ; Vol. 34, No. 13. pp. 2437-2449.
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