The histone chaperone TAF-I/SET/INHAT is required for transcription in vitro of chromatin templates

Matthew J. Gamble, Hediye Erdjument-Bromage, Paul Tempst, Leonard P. Freedman, Robert P. Fisher

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

To uncover factors required for transcription by RNA polymerase II on chromatin, we fractionated a mammalian cell nuclear extract. We identified the histone chaperone TAF-I (also known as INHAT [inhibitor of histone acetyltransferase]), which was previously proposed to repress transcription, as a potent activator of chromatin transcription responsive to the vitamin D 3 receptor or to Gal4-VP16. TAF-I associates with chromatin in vitro and can substitute for the related protein NAP-1 in assembling chromatin onto cloned DNA templates in cooperation with the remodeling enzyme ATP-dependent chromatin assembly factor (ACF). The chromatin assembly and transcriptional activation functions are distinct, however, and can be dissociated temporally. Efficient transcription of chromatin assembled with TAF-I still requires the presence of TAF-I during the polymerization reaction. Conversely, TAF-I cannot stimulate transcript elongation when added after the other factors necessary for assembly of a preinitiation complex on naked DNA. Thus, TAF-I is required to facilitate transcription at a step after chromatin assembly but before transcript elongation.

Original languageEnglish (US)
Pages (from-to)797-807
Number of pages11
JournalMolecular and Cellular Biology
Volume25
Issue number2
DOIs
StatePublished - Jan 2005
Externally publishedYes

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Histone Chaperones
Histone Acetyltransferases
Chromatin
Chromatin Assembly and Disassembly
Calcitriol Receptors
RNA Polymerase II
DNA
Cell Extracts
Polymerization
Transcriptional Activation
Transcription Factors
Adenosine Triphosphate
In Vitro Techniques
Enzymes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The histone chaperone TAF-I/SET/INHAT is required for transcription in vitro of chromatin templates. / Gamble, Matthew J.; Erdjument-Bromage, Hediye; Tempst, Paul; Freedman, Leonard P.; Fisher, Robert P.

In: Molecular and Cellular Biology, Vol. 25, No. 2, 01.2005, p. 797-807.

Research output: Contribution to journalArticle

Gamble, Matthew J. ; Erdjument-Bromage, Hediye ; Tempst, Paul ; Freedman, Leonard P. ; Fisher, Robert P. / The histone chaperone TAF-I/SET/INHAT is required for transcription in vitro of chromatin templates. In: Molecular and Cellular Biology. 2005 ; Vol. 25, No. 2. pp. 797-807.
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