The histone chaperone TAF-I/SET/INHAT is required for transcription in vitro of chromatin templates

Matthew J. Gamble, Hediye Erdjument-Bromage, Paul Tempst, Leonard P. Freedman, Robert P. Fisher

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Abstract

To uncover factors required for transcription by RNA polymerase II on chromatin, we fractionated a mammalian cell nuclear extract. We identified the histone chaperone TAF-I (also known as INHAT [inhibitor of histone acetyltransferase]), which was previously proposed to repress transcription, as a potent activator of chromatin transcription responsive to the vitamin D 3 receptor or to Gal4-VP16. TAF-I associates with chromatin in vitro and can substitute for the related protein NAP-1 in assembling chromatin onto cloned DNA templates in cooperation with the remodeling enzyme ATP-dependent chromatin assembly factor (ACF). The chromatin assembly and transcriptional activation functions are distinct, however, and can be dissociated temporally. Efficient transcription of chromatin assembled with TAF-I still requires the presence of TAF-I during the polymerization reaction. Conversely, TAF-I cannot stimulate transcript elongation when added after the other factors necessary for assembly of a preinitiation complex on naked DNA. Thus, TAF-I is required to facilitate transcription at a step after chromatin assembly but before transcript elongation.

Original languageEnglish (US)
Pages (from-to)797-807
Number of pages11
JournalMolecular and cellular biology
Volume25
Issue number2
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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