The high molecular weight form of endothelial cell von Willebrand factor is released by the regulated pathway

H. M. Tsai, R. L. Nagel, Victor Bernard Hatcher, A. C. Seaton, I. I. Sussman

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We have previously reported that two forms of von Willebrand factor (vWf) exist in cultured human umbilical vein endothelial cells: a high molecular weight (HMW) form that is released and can be proteolytically cleaved into a series of plasma-like multimers, and a nonsecreted low molecular weight (LMW) form. In this study, the mode of vWf release and the relationship between the two forms were examined. As determined by two-dimensional analysis as well as by immunoreactivity with an antibody to the propolypeptide, the LMW form of endothelial cell vWf consisted of a 260 kD pro-vWf polypeptide, while the HMW form consisted of a 225 kD mature polypeptide. Only the 260 kD polypeptide was susceptible to digestion with endoglycosidase H. Release of the HMW form into the culture media was accompanied by a decrease in cellular vWf. Treatment of endothelial cells with cycloheximide or tunicamycin caused a decrease in the LMW form but did not affect the secretion fo the HMW form. These results suggest that the two pools of vWf exist in endothelial cells - a LMW form of pro-vWf in the endoplasmic reticulum and a HMW form of mature vWf in the storage compartment. Release vWf derives only from the storage pool.

Original languageEnglish (US)
Pages (from-to)239-245
Number of pages7
JournalBritish Journal of Haematology
Volume79
Issue number2
StatePublished - 1991

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von Willebrand Factor
Endothelial Cells
Molecular Weight
Peptides
Tunicamycin
Glycoside Hydrolases
Human Umbilical Vein Endothelial Cells
Cycloheximide
Endoplasmic Reticulum
Culture Media
Digestion
Antibodies

ASJC Scopus subject areas

  • Hematology

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The high molecular weight form of endothelial cell von Willebrand factor is released by the regulated pathway. / Tsai, H. M.; Nagel, R. L.; Hatcher, Victor Bernard; Seaton, A. C.; Sussman, I. I.

In: British Journal of Haematology, Vol. 79, No. 2, 1991, p. 239-245.

Research output: Contribution to journalArticle

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