TY - JOUR
T1 - The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic
AU - Walbott, Hélène
AU - Machado-Pinilla, Rosario
AU - Liger, Dominique
AU - Blaud, Magali
AU - Réty, Stéphane
AU - Grozdanov, Petar N.
AU - Godin, Kate
AU - van Tilbeurgh, Herman
AU - Varani, Gabriele
AU - Thomas Meier, U.
AU - Leulliot, Nicolas
PY - 2011
Y1 - 2011
N2 - SHQ1 is an essential assembly factor for H/ACA ribonucleoproteins (RNPs) required for ribosome biogenesis, premRNA splicing, and telomere maintenance. SHQ1 binds dyskerin/NAP57, the catalytic subunit of human H/ACA RNPs, and this interaction is modulated by mutations causing X-linked dyskeratosis congenita. We report the crystal structure of the C-terminal domain of yeast SHQ1, Shq1p, and its complex with yeast dyskerin/NAP57, Cbf5p, lacking its catalytic domain. The C-terminal domain of Shq1p interacts with the RNA-binding domain of Cbf5p and, through structural mimicry, uses the RNA-protein-binding sites to achieve a specific protein-protein interface. We propose that Shq1p operates as a Cbf5p chaperone during RNP assembly by acting as an RNA placeholder, thereby preventing Cbf5p from nonspecific RNA binding before association with an H/ACA RNA and the other core RNP proteins.
AB - SHQ1 is an essential assembly factor for H/ACA ribonucleoproteins (RNPs) required for ribosome biogenesis, premRNA splicing, and telomere maintenance. SHQ1 binds dyskerin/NAP57, the catalytic subunit of human H/ACA RNPs, and this interaction is modulated by mutations causing X-linked dyskeratosis congenita. We report the crystal structure of the C-terminal domain of yeast SHQ1, Shq1p, and its complex with yeast dyskerin/NAP57, Cbf5p, lacking its catalytic domain. The C-terminal domain of Shq1p interacts with the RNA-binding domain of Cbf5p and, through structural mimicry, uses the RNA-protein-binding sites to achieve a specific protein-protein interface. We propose that Shq1p operates as a Cbf5p chaperone during RNP assembly by acting as an RNA placeholder, thereby preventing Cbf5p from nonspecific RNA binding before association with an H/ACA RNA and the other core RNP proteins.
KW - H/ACA RNPs
KW - Ribonucleoprotein assembly
KW - Telomerase
KW - X-linked dyskeratosis congenita
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U2 - 10.1101/gad.176834.111
DO - 10.1101/gad.176834.111
M3 - Article
C2 - 22085966
AN - SCOPUS:81255142796
SN - 0890-9369
VL - 25
SP - 23982408
JO - Genes and Development
JF - Genes and Development
IS - 22
ER -