The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic.

Hélène Walbott, Rosario Machado-Pinilla, Dominique Liger, Magali Blaud, Stéphane Réty, Petar N. Grozdanov, Kate Godin, Herman van Tilbeurgh, Gabriele Varani, U. Thomas Meier, Nicolas Leulliot

Research output: Contribution to journalArticle

Abstract

SHQ1 is an essential assembly factor for H/ACA ribonucleoproteins (RNPs) required for ribosome biogenesis, pre-mRNA splicing, and telomere maintenance. SHQ1 binds dyskerin/NAP57, the catalytic subunit of human H/ACA RNPs, and this interaction is modulated by mutations causing X-linked dyskeratosis congenita. We report the crystal structure of the C-terminal domain of yeast SHQ1, Shq1p, and its complex with yeast dyskerin/NAP57, Cbf5p, lacking its catalytic domain. The C-terminal domain of Shq1p interacts with the RNA-binding domain of Cbf5p and, through structural mimicry, uses the RNA-protein-binding sites to achieve a specific protein-protein interface. We propose that Shq1p operates as a Cbf5p chaperone during RNP assembly by acting as an RNA placeholder, thereby preventing Cbf5p from nonspecific RNA binding before association with an H/ACA RNA and the other core RNP proteins.

Original languageEnglish (US)
Pages (from-to)2398-2408
Number of pages11
JournalGenes & development
Volume25
Issue number22
StatePublished - Nov 15 2011
Externally publishedYes

Fingerprint

Ribonucleoproteins
RNA
Catalytic Domain
Proteins
Dyskeratosis Congenita
Yeasts
Complement Factor H
RNA Precursors
Telomere
Ribosomes
Binding Sites
Maintenance
Mutation
NAP57

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Walbott, H., Machado-Pinilla, R., Liger, D., Blaud, M., Réty, S., Grozdanov, P. N., ... Leulliot, N. (2011). The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic. Genes & development, 25(22), 2398-2408.

The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic. / Walbott, Hélène; Machado-Pinilla, Rosario; Liger, Dominique; Blaud, Magali; Réty, Stéphane; Grozdanov, Petar N.; Godin, Kate; van Tilbeurgh, Herman; Varani, Gabriele; Meier, U. Thomas; Leulliot, Nicolas.

In: Genes & development, Vol. 25, No. 22, 15.11.2011, p. 2398-2408.

Research output: Contribution to journalArticle

Walbott, H, Machado-Pinilla, R, Liger, D, Blaud, M, Réty, S, Grozdanov, PN, Godin, K, van Tilbeurgh, H, Varani, G, Meier, UT & Leulliot, N 2011, 'The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic.', Genes & development, vol. 25, no. 22, pp. 2398-2408.
Walbott H, Machado-Pinilla R, Liger D, Blaud M, Réty S, Grozdanov PN et al. The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic. Genes & development. 2011 Nov 15;25(22):2398-2408.
Walbott, Hélène ; Machado-Pinilla, Rosario ; Liger, Dominique ; Blaud, Magali ; Réty, Stéphane ; Grozdanov, Petar N. ; Godin, Kate ; van Tilbeurgh, Herman ; Varani, Gabriele ; Meier, U. Thomas ; Leulliot, Nicolas. / The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic. In: Genes & development. 2011 ; Vol. 25, No. 22. pp. 2398-2408.
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