The genome and structural proteome of an ocean siphovirus: A new window into the cyanobacterial 'mobilome'

Matthew B. Sullivan, Bryan Krastins, Jennifer L. Hughes, Libusha Kelly, Michael Chase, David Sarracino, Sallie W. Chisholm

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Prochlorococcus, an abundant phototroph in the oceans, are infected by members of three families of viruses: myo-, podo- and siphoviruses. Genomes of myo- and podoviruses isolated on Prochlorococcus contain DNA replication machinery and virion structural genes homologous to those from coliphages T4 and T7 respectively. They also contain a suite of genes of cyanobacterial origin, most notably photosynthesis genes, which are expressed during infection and appear integral to the evolutionary trajectory of both host and phage. Here we present the first genome of a cyanobacterial siphovirus, P-SS2, which was isolated from Atlantic slope waters using a Prochlorococcus host (MIT9313). The P-SS2 genome is larger than, and considerably divergent from, previously sequenced siphoviruses. It appears most closely related to lambdoid siphoviruses, with which it shares 13 functional homologues. The ∼108 kb P-SS2 genome encodes 131 predicted proteins and notably lacks photosynthesis genes which have consistently been found in other marine cyanophage, but does contain 14 other cyanobacterial homologues. While only six structural proteins were identified from the genome sequence, 35 proteins were detected experimentally; these mapped onto capsid and tail structural modules in the genome. P-SS2 is potentially capable of integration into its host as inferred from bioinformatically identified genetic machinery int, bet, exo and a 53 bp attachment site. The host attachment site appears to be a genomic island that is tied to insertion sequence (IS) activity that could facilitate mobility of a gene involved in the nitrogen-stress response. The homologous region and a secondary IS-element hot-spot in Synechococcus RS9917 are further evidence of IS-mediated genome evolution coincident with a probable relic prophage integration event. This siphovirus genome provides a glimpse into the biology of a deep-photic zone phage as well as the ocean cyanobacterial prophage and IS element 'mobilome'.

Original languageEnglish (US)
Pages (from-to)2935-2951
Number of pages17
JournalEnvironmental Microbiology
Volume11
Issue number11
DOIs
StatePublished - Nov 2009
Externally publishedYes

Fingerprint

Proteome
proteome
Oceans and Seas
genome
oceans
Genome
Prochlorococcus
ocean
transposons
gene
DNA Transposable Elements
Genes
Insertional Mutagenesis
Photosynthesis
machinery
bacteriophages
Bacteriophages
protein
photosynthesis
genes

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

The genome and structural proteome of an ocean siphovirus : A new window into the cyanobacterial 'mobilome'. / Sullivan, Matthew B.; Krastins, Bryan; Hughes, Jennifer L.; Kelly, Libusha; Chase, Michael; Sarracino, David; Chisholm, Sallie W.

In: Environmental Microbiology, Vol. 11, No. 11, 11.2009, p. 2935-2951.

Research output: Contribution to journalArticle

Sullivan, Matthew B. ; Krastins, Bryan ; Hughes, Jennifer L. ; Kelly, Libusha ; Chase, Michael ; Sarracino, David ; Chisholm, Sallie W. / The genome and structural proteome of an ocean siphovirus : A new window into the cyanobacterial 'mobilome'. In: Environmental Microbiology. 2009 ; Vol. 11, No. 11. pp. 2935-2951.
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