The gene for human erythrocyte protein 4.2 maps to chromosome 15q15

V. Najfeld, S. G. Ballard, J. Menninger, D. C. Ward, Eric E. Bouhassira, R. S. Schwartz, R. L. Nagel, A. C. Rybicki

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Protein 4.2 (P4.2), one of the major components of the red-blood-cell membrane, is located on the interior surface, where it binds with high affinity to the cytoplasmic domain of band 3. Individuals whose red blood cells are deficient in P4.2 have osmotically fragile, abnormally shaped cells and moderate hemolytic anemia. cDNA clones from both the 5′ and the 3′ coding regions of the P4.2 gene were used to map its chromosomal location by fluorescence in situ hybridization. The probes, individually or in combination, gave specific hybridization signals on chromosome 15. The hybridization locus was identified by combining fluorescence images of the probe signals with fluorescence banding patterns generated by Alu-PCR (R-like) probe and by DAPI staining (G-like). Our results demonstrate that the locus of the P4.2 gene is located within 15q15.

Original languageEnglish (US)
Pages (from-to)71-75
Number of pages5
JournalAmerican Journal of Human Genetics
Volume50
Issue number1
StatePublished - Jan 1992

Fingerprint

Chromosomes
Erythrocytes
Fluorescence
Genes
Chromosomes, Human, Pair 15
Proteins
Hemolytic Anemia
Fluorescence In Situ Hybridization
Open Reading Frames
Complementary DNA
Clone Cells
Cell Membrane
Staining and Labeling
Polymerase Chain Reaction
DAPI

ASJC Scopus subject areas

  • Genetics

Cite this

Najfeld, V., Ballard, S. G., Menninger, J., Ward, D. C., Bouhassira, E. E., Schwartz, R. S., ... Rybicki, A. C. (1992). The gene for human erythrocyte protein 4.2 maps to chromosome 15q15. American Journal of Human Genetics, 50(1), 71-75.

The gene for human erythrocyte protein 4.2 maps to chromosome 15q15. / Najfeld, V.; Ballard, S. G.; Menninger, J.; Ward, D. C.; Bouhassira, Eric E.; Schwartz, R. S.; Nagel, R. L.; Rybicki, A. C.

In: American Journal of Human Genetics, Vol. 50, No. 1, 01.1992, p. 71-75.

Research output: Contribution to journalArticle

Najfeld, V, Ballard, SG, Menninger, J, Ward, DC, Bouhassira, EE, Schwartz, RS, Nagel, RL & Rybicki, AC 1992, 'The gene for human erythrocyte protein 4.2 maps to chromosome 15q15', American Journal of Human Genetics, vol. 50, no. 1, pp. 71-75.
Najfeld V, Ballard SG, Menninger J, Ward DC, Bouhassira EE, Schwartz RS et al. The gene for human erythrocyte protein 4.2 maps to chromosome 15q15. American Journal of Human Genetics. 1992 Jan;50(1):71-75.
Najfeld, V. ; Ballard, S. G. ; Menninger, J. ; Ward, D. C. ; Bouhassira, Eric E. ; Schwartz, R. S. ; Nagel, R. L. ; Rybicki, A. C. / The gene for human erythrocyte protein 4.2 maps to chromosome 15q15. In: American Journal of Human Genetics. 1992 ; Vol. 50, No. 1. pp. 71-75.
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