The G protein coupled to the thromboxane A2 receptor in human platelets is a member of the novel G(q) family

A. Shenker, P. Goldsmith, C. G. Unson, A. M. Spiegel

Research output: Contribution to journalArticle

286 Scopus citations

Abstract

The thromboxane A2 (TXA2) receptor in human platelets is coupled to a pertussis toxin-insensitive G protein whose identity has remained unknown. Candidates for this role include the atypical G protein known as G(z) and members of a recently discovered G protein family known as G(q). Because of the proven utility of antibodies directed against the C terminus of G protein α subunits as functional probes, we prepared an antibody against a synthetic decapeptide corresponding to the C-terminal sequence shared by α11 and α(q), two members of the new family. This antibody (QL) does not recognize known α subunits but selectively binds to a 42-kDa protein in a variety of tissues, including human platelet membranes. QL and two other C-terminal antibodies, QN and AS, known to recognize α(z) and α(i2), respectively, were tested for their ability to block agonist-stimulated GTPase activity in human platelet membranes. Pretreatment of platelet membranes with AS has previously been shown to interfere with α2 adrenergic receptor-mediated inhibition of adenylylcyclase. As expected, only AS antibody produced inhibition of α2 receptor-stimulated GTPase. Pretreatment of membranes with QL, but not QN or AS, caused marked inhibition of TXA2 receptor-stimulated GTPase. This identifies the G protein coupled to human platelet TXA2 receptors as a member of the novel G(q) family.

Original languageEnglish (US)
Pages (from-to)9309-9313
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number14
StatePublished - Jul 22 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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