The G protein coupled to the thromboxane A2 receptor in human platelets is a member of the novel Gq family

Andrew Shenker, Paul Goldsmith, Cecilia G. Unson, Allen M. Spiegel

Research output: Contribution to journalArticle

281 Citations (Scopus)

Abstract

The thromboxane A2 (TXA2) receptor in human platelets is coupled to a pertussis toxin-insensitive G protein whose identity has remained unknown. Candidates for this role include the atypical G protein known as Gz and members of a recently discovered G protein family known as Gq. Because of the proven utility of antibodies directed against the C terminus of G protein α subunits as functional probes, we prepared an antibody against a synthetic decapeptide corresponding to the C-terminal sequence shared by an and αq, two members of the new family. This antibody (QL) does not recognize known a subunits but selectively binds to a 42-kDa protein in a variety of tissues, including human platelet membranes. QL and two other C-terminal antibodies, QN and AS, known to recognize αz and αi2, respectively, were tested for their ability to block agonist-stimulated GTPase activity in human platelet membranes. Pretreatment of platelet membranes with AS has previously been shown to interfere with α2 adrenergic receptor-mediated inhibition of adenylylcyclase. As expected, only AS antibody produced inhibition of α2 receptor-stimulated GTPase. Pretreatment of membranes with QL, but not QN or AS, caused marked inhibition of TXA2 receptor-stimulated GTPase. This identifies the G protein coupled to human platelet TXAz receptors as a member of the novel Gq family.

Original languageEnglish (US)
Pages (from-to)9309-9313
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number14
StatePublished - 1991
Externally publishedYes

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Prostaglandin H2 Receptors Thromboxane A2
Platelets
GTP-Binding Proteins
Blood Platelets
GTP Phosphohydrolases
Antibodies
Membranes
Aptitude
Pertussis Toxin
Protein Subunits
Human Activities
Adrenergic Receptors
Tissue
Inhibition (Psychology)
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

The G protein coupled to the thromboxane A2 receptor in human platelets is a member of the novel Gq family. / Shenker, Andrew; Goldsmith, Paul; Unson, Cecilia G.; Spiegel, Allen M.

In: Journal of Biological Chemistry, Vol. 266, No. 14, 1991, p. 9309-9313.

Research output: Contribution to journalArticle

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