The Fusarium oxysporum cell wall proteome under adhesion-inducing conditions

Rafael Prados-Rosales, Jose L. Luque-Garcia, Raquel Martínez-López, Concha Gil, Antonio Di Pietro

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Fusarium oxysporum is a soilborne fungus that causes vascular wilt disease on a wide range of crops. During initial stages of infection, fungal hyphae attach firmly to roots, penetrate the cortex and colonize xylem vessels. The mechanisms underlying root attachment are poorly understood, although it was previously shown that this process depends on Fmk1, a mitogen-activated protein kinase orthologous to the mating/filamentation mitogen-activated protein kinases Fus3/Kss1 in yeast. We investigated the hypothesis that root adhesion is mediated by fungal cell wall proteins (CWPs). To characterize the cell wall subproteome of F. oxysporum, we performed LC-MS/MS analysis of tryptic digests of purified cell walls obtained from adhesion-inducing conditions, identifying a total of 174 proteins, 19 of which contain a predicted signal peptide and 10 of which have a predicted glycosylphosphatidyl-inositol motif. 2-D DIGE was used to compare four different fractions of CWPs extracted from hyphae of the wild-type strain and the Dfmk1 mutant. We detected 18 proteins differing significantly in abundance between the two strains. Differential expression of five of these proteins was confirmed by RT-PCR analysis. A significant fraction of the subproteome lacked functional information, highlighting the limitations in the current understanding of CWPs in F. oxysporum.

Original languageEnglish (US)
Pages (from-to)4755-4769
Number of pages15
JournalProteomics
Volume9
Issue number20
DOIs
StatePublished - Oct 20 2009

Keywords

  • Adhesion
  • Cell wall
  • Fusarium
  • Microbiology
  • Mitogen-activated protein kinase
  • Virulence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'The Fusarium oxysporum cell wall proteome under adhesion-inducing conditions'. Together they form a unique fingerprint.

  • Cite this

    Prados-Rosales, R., Luque-Garcia, J. L., Martínez-López, R., Gil, C., & Di Pietro, A. (2009). The Fusarium oxysporum cell wall proteome under adhesion-inducing conditions. Proteomics, 9(20), 4755-4769. https://doi.org/10.1002/pmic.200800950