The formin DAAM functions as molecular effector of the planar cell polarity pathway during axonal development in Drosophila

Rita Gombos, Ede Migh, Otilia Antal, Anindita Mukherjee, Andreas Jenny, József Mihály

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recent studies established that the planar cell polarity (PCP) pathway is critical for various aspects of nervous system development and function, including axonal guidance. Although it seems clear thatPCPsignaling regulates actin dynamics, the mechanisms through which this occurs remain elusive. Here, we establish a functional link between the PCP system and one specific actin regulator, the formin DAAM, which has previously been shown to be required for embryonic axonal morphogenesis and filopodia formation in the growth cone.Weshow that dDAAM also plays a pivotal role during axonal growth and guidance in the adult Drosophila mushroom body, a brain center for learning and memory. By using a combination of genetic and biochemical assays, we demonstrate that Wnt5 and the PCP signaling proteins Frizzled, Strabismus, and Dishevelled act in concert with the small GTPase Rac1 to activate the actin assembly functions of dDAAM essential for correct targeting of mushroom body axons. Collectively, these data suggest that dDAAM is used as a major molecular effector of the PCP guidance pathway. By uncovering a signaling system from the Wnt5 guidance cue to an actin assembly factor, we propose that the Wnt5/PCP navigation system is linked by dDAAM to the regulation of the growth cone actin cytoskeleton, and thereby growth cone behavior, in a direct way.

Original languageEnglish (US)
Pages (from-to)10154-10167
Number of pages14
JournalJournal of Neuroscience
Volume35
Issue number28
DOIs
StatePublished - Jul 15 2015

Fingerprint

Cell Polarity
Drosophila
Growth Cones
Actins
Mushroom Bodies
Frizzled Receptors
Pseudopodia
Critical Pathways
Monomeric GTP-Binding Proteins
Strabismus
Actin Cytoskeleton
Morphogenesis
Nervous System
Cues
Axons
Molecular Biology
Learning
Brain
Growth

Keywords

  • Axon growth
  • DDAAM
  • Drosophila
  • Formin
  • Mushroom body
  • PCP

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The formin DAAM functions as molecular effector of the planar cell polarity pathway during axonal development in Drosophila. / Gombos, Rita; Migh, Ede; Antal, Otilia; Mukherjee, Anindita; Jenny, Andreas; Mihály, József.

In: Journal of Neuroscience, Vol. 35, No. 28, 15.07.2015, p. 10154-10167.

Research output: Contribution to journalArticle

Gombos, Rita ; Migh, Ede ; Antal, Otilia ; Mukherjee, Anindita ; Jenny, Andreas ; Mihály, József. / The formin DAAM functions as molecular effector of the planar cell polarity pathway during axonal development in Drosophila. In: Journal of Neuroscience. 2015 ; Vol. 35, No. 28. pp. 10154-10167.
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