The fate of HIV-1 capsid: A biochemical assay for HIV-1 uncoating

Yang Yang, Jeremy Luban, Felipe Diaz-Griffero

Research output: Chapter in Book/Report/Conference proceedingChapter

24 Scopus citations

Abstract

The uncoating process of HIV-1 is a poorly understood process, so the development of a reliable assay to study uncoating is critical for moving the field forward. Here we describe an uncoating assay that currently represents the state-of-the-art biochemical procedure for monitoring uncoating and core stability during infection. This assay is based on the biochemical separation of soluble capsid protein from particulate capsid cores and provides information about the fate of the capsid during infection.

Original languageEnglish (US)
Title of host publicationHuman Retroviruses
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages29-36
Number of pages8
ISBN (Print)9781627036696
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1087
ISSN (Print)1064-3745

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Keywords

  • Capsid
  • HIV-1 core
  • Sucrose cushion
  • TRIM5α
  • Uncoating

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yang, Y., Luban, J., & Diaz-Griffero, F. (2014). The fate of HIV-1 capsid: A biochemical assay for HIV-1 uncoating. In Human Retroviruses: Methods and Protocols (pp. 29-36). (Methods in Molecular Biology; Vol. 1087). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-670-2_3