Because HER-2/neu overexpression is important in cancer development, we looked for a method of suppressing the cell transformation mediated by HER- 2/neu overexpression. We have identified that the DNA-binding protein PEA3, which is encoded by a previously isolated gene of the ets family, specifically targeted a DNA sequence on the HER-2/neu promoter and downregulated the promoter activity. Expression of PEA3 resulted in preferential inhibition of cell growth and tumor development of HER-2/neu- overexpressing cancer cells. This is a new approach to targeting HER-2/neu overexpression and also provides a rationale to the design for repressors of diseases caused by overexpression of pathogenic genes.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)