The Ets factor Spi-B is a direct critical target of the coactivator OBF-1

Boris Bartholdy, Camille Du Roure, Alain Bordon, Dianne Emslie, Lynn M. Corcoran, Patrick Matthias

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

OBF-1 (Bob.1, OCA-B) is a lymphoid-specific transcriptional coactivator that associates with the transcription factors Oct-1 or Oct-2 on the conserved octamer element present in the promoters of several ubiquitous and lymphoid-specific genes. OBF-1-deficient mice have B cell-intrinsic defects, lack germinal centers, and have severely impaired immune responses to T cell-dependent antigens. Crucial genes that are regulated by OBF-1 and that might explain the observed phenotype of OBF-1 deficiency have remained elusive to date. Here we have generated transgenic mice expressing OBF-1 specifically in T cells and examined these together with mice lacking OBF-1 to discover transcriptional targets of this coactivator. Using microarray analysis, we have identified the Ets transcription factor Spi-B as a direct target gene critically regulated by OBF-1 that can help explain the phenotype of OBF-1-deficient mice. Spi-B has been implicated in signaling pathways downstream of the B cell receptor and is essential for germinal center formation and maintenance. The present findings establish a hierarchy between these two factors and provide a molecular link between OBF-1 and B cell receptor signaling.

Original languageEnglish (US)
Pages (from-to)11665-11670
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number31
DOIs
StatePublished - Aug 1 2006
Externally publishedYes

Keywords

  • B cell development
  • Gene regulation
  • Transcription factors
  • Transgenic mice

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'The Ets factor Spi-B is a direct critical target of the coactivator OBF-1'. Together they form a unique fingerprint.

  • Cite this