The energetic linkage of GTP hydrolysis and the synthesis of activated sulfate

Changxian Liu, Ya Suo, Thomas S. Leyh

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

ATP sulfurylase, from Escherichia coli K-12, catalyzes both the hydrolysis of GTP and the synthesis of activated sulfate (APS). This paper describes the energetic linkage of these reactions and the events that couple them. Steady-state and single-turnover experiments suggest that the binding of GTP inhibits APS production and that the hydrolysis of GTP is required to generate the enzyme form(s) that produces APS. It is this progression from the inhibitory, E·GTP, to the productive, E-GDP, complexes in the cycle of APS synthesis that energetically links these two reactions. This model stands in contrast to other GTPase/target systems in which the binding of GTP alone is sufficient to catalyze multiple turnovers of the target reaction. The stoichiometry of GTP hydrolysis to APS synthesis is 1:1, and equilibrium measurements show that -9.1 kcal/mol, produced by the hydrolysis of GTP, is used to thermodynamically drive production of APS and PPi. These findings establish the mechanism of energy transfer in this novel GTPase/target system, and substantially alter our understanding of the energetics of sulfate activation, an essential step in the metabolic assimilation of sulfur.

Original languageEnglish (US)
Pages (from-to)7309-7314
Number of pages6
JournalBiochemistry
Volume33
Issue number23
StatePublished - 1994

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Guanosine Triphosphate
Sulfates
Hydrolysis
GTP Phosphohydrolases
Sulfate Adenylyltransferase
Energy Transfer
Sulfur
Stoichiometry
Energy transfer
Escherichia coli
Chemical activation
Enzymes
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

The energetic linkage of GTP hydrolysis and the synthesis of activated sulfate. / Liu, Changxian; Suo, Ya; Leyh, Thomas S.

In: Biochemistry, Vol. 33, No. 23, 1994, p. 7309-7314.

Research output: Contribution to journalArticle

Liu, Changxian ; Suo, Ya ; Leyh, Thomas S. / The energetic linkage of GTP hydrolysis and the synthesis of activated sulfate. In: Biochemistry. 1994 ; Vol. 33, No. 23. pp. 7309-7314.
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