Abstract
Previously we have shown that growth of melanized fungi is stimulated by low levels of gamma radiation. The goal of this study was to examine the effects of visible light, UV light, and gamma radiation on the energy level (ATP concentration) in melanized Cryptococcus neoformans cells. Melanized C. neoformans cells as well as non-melanized controls were subjected to visible, UV or gamma radiation, and ATP was quantified by measuring the amount of light emitted by the ATP-dependent reaction of luciferase with luciferin. We found that all three forms of radiation led to a reduction in the ATP levels in melanized C. neoformans cells. This points to a universal melanin-related mechanism underlying observation of ATP decrease in irradiated melanized cells. In contrast, in non-melanized cells visible light led to increase in ATP levels; gamma radiation did not cause any changes while UV exposure resulted in some ATP decrease, however, much less pronounced than in melanized cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 945-949 |
| Number of pages | 5 |
| Journal | Fungal Biology |
| Volume | 115 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2011 |
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Keywords
- ATP
- Cryptococcus neoformans
- Ionizing radiation
- Melanin
- UV
- Visible light
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Plant Science
- Genetics
- Infectious Diseases
Cite this
The effects of gamma radiation, UV and visible light on ATP levels in yeast cells depend on cellular melanization. / Bryan, Ruth; Jiang, Zewei; Friedman, Matthew; Dadachova, Ekaterina.
In: Fungal Biology, Vol. 115, No. 10, 10.2011, p. 945-949.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effects of gamma radiation, UV and visible light on ATP levels in yeast cells depend on cellular melanization
AU - Bryan, Ruth
AU - Jiang, Zewei
AU - Friedman, Matthew
AU - Dadachova, Ekaterina
PY - 2011/10
Y1 - 2011/10
N2 - Previously we have shown that growth of melanized fungi is stimulated by low levels of gamma radiation. The goal of this study was to examine the effects of visible light, UV light, and gamma radiation on the energy level (ATP concentration) in melanized Cryptococcus neoformans cells. Melanized C. neoformans cells as well as non-melanized controls were subjected to visible, UV or gamma radiation, and ATP was quantified by measuring the amount of light emitted by the ATP-dependent reaction of luciferase with luciferin. We found that all three forms of radiation led to a reduction in the ATP levels in melanized C. neoformans cells. This points to a universal melanin-related mechanism underlying observation of ATP decrease in irradiated melanized cells. In contrast, in non-melanized cells visible light led to increase in ATP levels; gamma radiation did not cause any changes while UV exposure resulted in some ATP decrease, however, much less pronounced than in melanized cells.
AB - Previously we have shown that growth of melanized fungi is stimulated by low levels of gamma radiation. The goal of this study was to examine the effects of visible light, UV light, and gamma radiation on the energy level (ATP concentration) in melanized Cryptococcus neoformans cells. Melanized C. neoformans cells as well as non-melanized controls were subjected to visible, UV or gamma radiation, and ATP was quantified by measuring the amount of light emitted by the ATP-dependent reaction of luciferase with luciferin. We found that all three forms of radiation led to a reduction in the ATP levels in melanized C. neoformans cells. This points to a universal melanin-related mechanism underlying observation of ATP decrease in irradiated melanized cells. In contrast, in non-melanized cells visible light led to increase in ATP levels; gamma radiation did not cause any changes while UV exposure resulted in some ATP decrease, however, much less pronounced than in melanized cells.
KW - ATP
KW - Cryptococcus neoformans
KW - Ionizing radiation
KW - Melanin
KW - UV
KW - Visible light
UR - http://www.scopus.com/inward/record.url?scp=80053211461&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053211461&partnerID=8YFLogxK
U2 - 10.1016/j.funbio.2011.04.003
DO - 10.1016/j.funbio.2011.04.003
M3 - Article
C2 - 21944206
AN - SCOPUS:80053211461
VL - 115
SP - 945
EP - 949
JO - Fungal Biology
JF - Fungal Biology
SN - 1878-6146
IS - 10
ER -