The effects of gamma radiation, UV and visible light on ATP levels in yeast cells depend on cellular melanization

Ruth Bryan, Zewei Jiang, Matthew Friedman, Ekaterina Dadachova

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Previously we have shown that growth of melanized fungi is stimulated by low levels of gamma radiation. The goal of this study was to examine the effects of visible light, UV light, and gamma radiation on the energy level (ATP concentration) in melanized Cryptococcus neoformans cells. Melanized C. neoformans cells as well as non-melanized controls were subjected to visible, UV or gamma radiation, and ATP was quantified by measuring the amount of light emitted by the ATP-dependent reaction of luciferase with luciferin. We found that all three forms of radiation led to a reduction in the ATP levels in melanized C. neoformans cells. This points to a universal melanin-related mechanism underlying observation of ATP decrease in irradiated melanized cells. In contrast, in non-melanized cells visible light led to increase in ATP levels; gamma radiation did not cause any changes while UV exposure resulted in some ATP decrease, however, much less pronounced than in melanized cells.

Original languageEnglish (US)
Pages (from-to)945-949
Number of pages5
JournalFungal Biology
Volume115
Issue number10
DOIs
StatePublished - Oct 2011

Fingerprint

melanization
Gamma Rays
Ultraviolet Rays
gamma radiation
yeast
ultraviolet radiation
Adenosine Triphosphate
Yeasts
yeasts
Light
Cryptococcus neoformans
cells
melanin
luciferin
fungus
luciferase
Melanins
effect
Luciferases
Fungi

Keywords

  • ATP
  • Cryptococcus neoformans
  • Ionizing radiation
  • Melanin
  • UV
  • Visible light

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Genetics
  • Infectious Diseases

Cite this

The effects of gamma radiation, UV and visible light on ATP levels in yeast cells depend on cellular melanization. / Bryan, Ruth; Jiang, Zewei; Friedman, Matthew; Dadachova, Ekaterina.

In: Fungal Biology, Vol. 115, No. 10, 10.2011, p. 945-949.

Research output: Contribution to journalArticle

Bryan, Ruth ; Jiang, Zewei ; Friedman, Matthew ; Dadachova, Ekaterina. / The effects of gamma radiation, UV and visible light on ATP levels in yeast cells depend on cellular melanization. In: Fungal Biology. 2011 ; Vol. 115, No. 10. pp. 945-949.
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