The effects of flap ischemia on normal and diabetic progenitor cell function

Rica Tanaka, Mika Wada, Sang Mo Kwon, Haruchika Masuda, Jacquelyn Carr, Rie Ito, Muneo Miyasaka, Stephen M. Warren, Takayuki Asahara, Oren M. Tepper

Research output: Contribution to journalArticle

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Abstract

BACKGROUND: Endothelial progenitor cells play an important role in neovascularization of ischemic flaps, a process that is significantly impaired in diabetes. This is the first investigation into the effects of flap ischemia on circulating and bone marrow-derived endothelial progenitor cells. Potential mechanisms for impaired vasculogenesis in diabetes are also investigated. METHODS: Circulating and bone marrow-derived endothelial progenitor cells were isolated from wild-type (n = 24) and diabetic mice (n = 24) with ischemic flaps (days 0, 1, 3, and 7). The number and vasculogenic function of primitive and definitive endothelial progenitor cells were determined by fluorescence- activated cell sorting analysis, culture assay, and vasculogenic colony-forming assay. RESULTS: Ischemia mobilized endothelial progenitor cells (25 ± 0.5 cells per high-power field at day 7 versus 9.0 ± 0.6 cells per high-power field, p < 0.01) and enhanced the vasculogenic potential of circulating primitive endothelial progenitor cells (23 ± 3.2 at day 3 versus 14 ± 0.8, p < 0.01) relative to baseline. In the bone marrow, endothelial progenitor cell number and vasculogenic potential peaked at day 3 (2.1 ± 0.3 × 10 cells versus 1.3 ± 0.1 × 10 cells, p < 0.05; 36 ± 1.9 versus 27 ± 1.6, p < 0.05, respectively). In diabetes, circulating endothelial progenitor cell mobilization (5.8 ± 0.4 cells per high-power field versus 9.0 ± 0.6 cells per high-power field, p < 0.01) and vasculogenic potential (36 ± 1.7 versus 43 ± 2.6, p < 0.05) were impaired relative to the wild-type animals. Bone marrow-derived endothelial progenitor cell number was normal in diabetic animals, but the vasculogenic potential of these cells was significantly impaired (5.7 ± 0.8 day 1 versus 13.4 ± 2.5, p < 0.05). CONCLUSIONS: Flap ischemia induces phenotypic changes in bone marrow-derived endothelial progenitor cells that subsequently traffic through the circulation. The vasculogenic potential of endothelial progenitor cells at various stages of differentiation is impaired in diabetes and thus may account for impaired ischemia-induced vasculogenesis observed clinically.

Original languageEnglish (US)
Pages (from-to)1929-1942
Number of pages14
JournalPlastic and Reconstructive Surgery
Volume121
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

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Stem Cells
Ischemia
Bone Marrow
Endothelial Progenitor Cells
Cell Count
Wild Animals
Flow Cytometry

ASJC Scopus subject areas

  • Surgery

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The effects of flap ischemia on normal and diabetic progenitor cell function. / Tanaka, Rica; Wada, Mika; Kwon, Sang Mo; Masuda, Haruchika; Carr, Jacquelyn; Ito, Rie; Miyasaka, Muneo; Warren, Stephen M.; Asahara, Takayuki; Tepper, Oren M.

In: Plastic and Reconstructive Surgery, Vol. 121, No. 6, 06.2008, p. 1929-1942.

Research output: Contribution to journalArticle

Tanaka, R, Wada, M, Kwon, SM, Masuda, H, Carr, J, Ito, R, Miyasaka, M, Warren, SM, Asahara, T & Tepper, OM 2008, 'The effects of flap ischemia on normal and diabetic progenitor cell function', Plastic and Reconstructive Surgery, vol. 121, no. 6, pp. 1929-1942. https://doi.org/10.1097/PRS.0b013e3181715218
Tanaka, Rica ; Wada, Mika ; Kwon, Sang Mo ; Masuda, Haruchika ; Carr, Jacquelyn ; Ito, Rie ; Miyasaka, Muneo ; Warren, Stephen M. ; Asahara, Takayuki ; Tepper, Oren M. / The effects of flap ischemia on normal and diabetic progenitor cell function. In: Plastic and Reconstructive Surgery. 2008 ; Vol. 121, No. 6. pp. 1929-1942.
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abstract = "BACKGROUND: Endothelial progenitor cells play an important role in neovascularization of ischemic flaps, a process that is significantly impaired in diabetes. This is the first investigation into the effects of flap ischemia on circulating and bone marrow-derived endothelial progenitor cells. Potential mechanisms for impaired vasculogenesis in diabetes are also investigated. METHODS: Circulating and bone marrow-derived endothelial progenitor cells were isolated from wild-type (n = 24) and diabetic mice (n = 24) with ischemic flaps (days 0, 1, 3, and 7). The number and vasculogenic function of primitive and definitive endothelial progenitor cells were determined by fluorescence- activated cell sorting analysis, culture assay, and vasculogenic colony-forming assay. RESULTS: Ischemia mobilized endothelial progenitor cells (25 ± 0.5 cells per high-power field at day 7 versus 9.0 ± 0.6 cells per high-power field, p < 0.01) and enhanced the vasculogenic potential of circulating primitive endothelial progenitor cells (23 ± 3.2 at day 3 versus 14 ± 0.8, p < 0.01) relative to baseline. In the bone marrow, endothelial progenitor cell number and vasculogenic potential peaked at day 3 (2.1 ± 0.3 × 10 cells versus 1.3 ± 0.1 × 10 cells, p < 0.05; 36 ± 1.9 versus 27 ± 1.6, p < 0.05, respectively). In diabetes, circulating endothelial progenitor cell mobilization (5.8 ± 0.4 cells per high-power field versus 9.0 ± 0.6 cells per high-power field, p < 0.01) and vasculogenic potential (36 ± 1.7 versus 43 ± 2.6, p < 0.05) were impaired relative to the wild-type animals. Bone marrow-derived endothelial progenitor cell number was normal in diabetic animals, but the vasculogenic potential of these cells was significantly impaired (5.7 ± 0.8 day 1 versus 13.4 ± 2.5, p < 0.05). CONCLUSIONS: Flap ischemia induces phenotypic changes in bone marrow-derived endothelial progenitor cells that subsequently traffic through the circulation. The vasculogenic potential of endothelial progenitor cells at various stages of differentiation is impaired in diabetes and thus may account for impaired ischemia-induced vasculogenesis observed clinically.",
author = "Rica Tanaka and Mika Wada and Kwon, {Sang Mo} and Haruchika Masuda and Jacquelyn Carr and Rie Ito and Muneo Miyasaka and Warren, {Stephen M.} and Takayuki Asahara and Tepper, {Oren M.}",
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T1 - The effects of flap ischemia on normal and diabetic progenitor cell function

AU - Tanaka, Rica

AU - Wada, Mika

AU - Kwon, Sang Mo

AU - Masuda, Haruchika

AU - Carr, Jacquelyn

AU - Ito, Rie

AU - Miyasaka, Muneo

AU - Warren, Stephen M.

AU - Asahara, Takayuki

AU - Tepper, Oren M.

PY - 2008/6

Y1 - 2008/6

N2 - BACKGROUND: Endothelial progenitor cells play an important role in neovascularization of ischemic flaps, a process that is significantly impaired in diabetes. This is the first investigation into the effects of flap ischemia on circulating and bone marrow-derived endothelial progenitor cells. Potential mechanisms for impaired vasculogenesis in diabetes are also investigated. METHODS: Circulating and bone marrow-derived endothelial progenitor cells were isolated from wild-type (n = 24) and diabetic mice (n = 24) with ischemic flaps (days 0, 1, 3, and 7). The number and vasculogenic function of primitive and definitive endothelial progenitor cells were determined by fluorescence- activated cell sorting analysis, culture assay, and vasculogenic colony-forming assay. RESULTS: Ischemia mobilized endothelial progenitor cells (25 ± 0.5 cells per high-power field at day 7 versus 9.0 ± 0.6 cells per high-power field, p < 0.01) and enhanced the vasculogenic potential of circulating primitive endothelial progenitor cells (23 ± 3.2 at day 3 versus 14 ± 0.8, p < 0.01) relative to baseline. In the bone marrow, endothelial progenitor cell number and vasculogenic potential peaked at day 3 (2.1 ± 0.3 × 10 cells versus 1.3 ± 0.1 × 10 cells, p < 0.05; 36 ± 1.9 versus 27 ± 1.6, p < 0.05, respectively). In diabetes, circulating endothelial progenitor cell mobilization (5.8 ± 0.4 cells per high-power field versus 9.0 ± 0.6 cells per high-power field, p < 0.01) and vasculogenic potential (36 ± 1.7 versus 43 ± 2.6, p < 0.05) were impaired relative to the wild-type animals. Bone marrow-derived endothelial progenitor cell number was normal in diabetic animals, but the vasculogenic potential of these cells was significantly impaired (5.7 ± 0.8 day 1 versus 13.4 ± 2.5, p < 0.05). CONCLUSIONS: Flap ischemia induces phenotypic changes in bone marrow-derived endothelial progenitor cells that subsequently traffic through the circulation. The vasculogenic potential of endothelial progenitor cells at various stages of differentiation is impaired in diabetes and thus may account for impaired ischemia-induced vasculogenesis observed clinically.

AB - BACKGROUND: Endothelial progenitor cells play an important role in neovascularization of ischemic flaps, a process that is significantly impaired in diabetes. This is the first investigation into the effects of flap ischemia on circulating and bone marrow-derived endothelial progenitor cells. Potential mechanisms for impaired vasculogenesis in diabetes are also investigated. METHODS: Circulating and bone marrow-derived endothelial progenitor cells were isolated from wild-type (n = 24) and diabetic mice (n = 24) with ischemic flaps (days 0, 1, 3, and 7). The number and vasculogenic function of primitive and definitive endothelial progenitor cells were determined by fluorescence- activated cell sorting analysis, culture assay, and vasculogenic colony-forming assay. RESULTS: Ischemia mobilized endothelial progenitor cells (25 ± 0.5 cells per high-power field at day 7 versus 9.0 ± 0.6 cells per high-power field, p < 0.01) and enhanced the vasculogenic potential of circulating primitive endothelial progenitor cells (23 ± 3.2 at day 3 versus 14 ± 0.8, p < 0.01) relative to baseline. In the bone marrow, endothelial progenitor cell number and vasculogenic potential peaked at day 3 (2.1 ± 0.3 × 10 cells versus 1.3 ± 0.1 × 10 cells, p < 0.05; 36 ± 1.9 versus 27 ± 1.6, p < 0.05, respectively). In diabetes, circulating endothelial progenitor cell mobilization (5.8 ± 0.4 cells per high-power field versus 9.0 ± 0.6 cells per high-power field, p < 0.01) and vasculogenic potential (36 ± 1.7 versus 43 ± 2.6, p < 0.05) were impaired relative to the wild-type animals. Bone marrow-derived endothelial progenitor cell number was normal in diabetic animals, but the vasculogenic potential of these cells was significantly impaired (5.7 ± 0.8 day 1 versus 13.4 ± 2.5, p < 0.05). CONCLUSIONS: Flap ischemia induces phenotypic changes in bone marrow-derived endothelial progenitor cells that subsequently traffic through the circulation. The vasculogenic potential of endothelial progenitor cells at various stages of differentiation is impaired in diabetes and thus may account for impaired ischemia-induced vasculogenesis observed clinically.

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