TY - JOUR
T1 - The effects of chronic exposure to supraphysiological concentrations of 3,5,3′ triiodo‐L‐thyronine (T3) on cultured GC cells
AU - Reynolds, Anne Marie
AU - Surks, Martin I.
AU - Shapiro, Lawrence E.
PY - 1991/12
Y1 - 1991/12
N2 - We have characterized the effects of supraphysiological concentrations of T3 on GC cells, a cultured cell line in which physiologic concentrations of T3 regulate cell growth, protein content, and growth hormone (GH) production. GC cells were exposed to 3 times (1.0 nM) and 80 times (25.0 nM) the physiologic concentration of T3 (0.3 nM) for either 4 d or for greater than 3 months. Both short and prolonged exposure to supranormal T3 concentrations supported maximal cell growth rate and induced significant increases in total protein (P < 0.025) and GH production (P < 0.01) per celi when compared to measurements in control GC cells. In addition, exposure to 1.0 nM and 25.0 nM T3 for greater than 3 months enhanced the toxicity of heat shock in a manner similar to previously described effects on GC cells due to T3 exposure of shorter duration. Thus, initial responses to raised T3 concentrations in cultured GC cells persisted without alteration when hormone exposure was prolonged for greater than 3 months.
AB - We have characterized the effects of supraphysiological concentrations of T3 on GC cells, a cultured cell line in which physiologic concentrations of T3 regulate cell growth, protein content, and growth hormone (GH) production. GC cells were exposed to 3 times (1.0 nM) and 80 times (25.0 nM) the physiologic concentration of T3 (0.3 nM) for either 4 d or for greater than 3 months. Both short and prolonged exposure to supranormal T3 concentrations supported maximal cell growth rate and induced significant increases in total protein (P < 0.025) and GH production (P < 0.01) per celi when compared to measurements in control GC cells. In addition, exposure to 1.0 nM and 25.0 nM T3 for greater than 3 months enhanced the toxicity of heat shock in a manner similar to previously described effects on GC cells due to T3 exposure of shorter duration. Thus, initial responses to raised T3 concentrations in cultured GC cells persisted without alteration when hormone exposure was prolonged for greater than 3 months.
UR - http://www.scopus.com/inward/record.url?scp=0025720482&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025720482&partnerID=8YFLogxK
U2 - 10.1002/jcp.1041490325
DO - 10.1002/jcp.1041490325
M3 - Article
C2 - 1744179
AN - SCOPUS:0025720482
SN - 0021-9541
VL - 149
SP - 544
EP - 547
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 3
ER -