The Effects of Alpha Interferon on the Development of Autoimmune Thyroiditis in the NOD H2h4 Mouse

Yael Oppenheim, Grace Kim, Yoshiyuki Ban, Pamela Unger, Erlinda Concepcion, Takao Ando, Yaron Tomer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Alpha interferon (αIFN) therapy is known to induce thyroid autoimmunity in up to 40% of patients. The mechanism is unknown, but Th1 switching has been hypothesized. The aim of our study was to examine whether αIFN accelerated the development of thyroiditis in genetically susceptible mice. We took advantage of NOD-H2h4, a genetically susceptible animal model, which develops thyroiditis when fed a high iodine diet. Six to eight week old male NOD H2h4 mice were injected with mouse αIFN (200 units) or with saline three times a week for 8 weeks. All mice drank iodinated water (0.15%). Mice were sacrificed after 8 weeks of injection. Their thyroids were examined for histology and blood was tested for antithyroglobulin antibody levels. T4 and glucose levels were also assessed. In the IFN-injected group, 6/13 (46.2%) developed thyroiditis and/or thyroid antibodies while in the saline-injected group, only 4/13 (30.8%) developed thyroiditis and/or thyroid antibodies (p = 0.4). The grade of thyroiditis was not different amongst the two groups. None of the mice developed clinical thyroiditis or diabetes mellitus. Our results showed that αIFN treatment did not accelerate thyroiditis in this mouse model. This may imply that αIFN induces thyroiditis in a non-genetically dependent manner, and this would not be detected in a genetically susceptible mouse model if the effect were small. Alternatively, it is possible that αIFN did not induce thyroiditis in mice because, unlike in humans, in mice αIFN does not induce Th1 switching.

Original languageEnglish (US)
Pages (from-to)161-165
Number of pages5
JournalClinical and Developmental Immunology
Volume10
Issue number2-4
DOIs
StatePublished - Jun 2003
Externally publishedYes

Fingerprint

Autoimmune Thyroiditis
Thyroiditis
Inbred NOD Mouse
Interferon-alpha
Interferons
Thyroid Gland
Antibodies
Autoimmunity
Iodine
Histology
Diabetes Mellitus
Animal Models
Diet
Glucose
Injections

Keywords

  • Alpha interferon
  • Animal model
  • Autoimmunity
  • Thyroiditis

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Developmental Biology

Cite this

The Effects of Alpha Interferon on the Development of Autoimmune Thyroiditis in the NOD H2h4 Mouse. / Oppenheim, Yael; Kim, Grace; Ban, Yoshiyuki; Unger, Pamela; Concepcion, Erlinda; Ando, Takao; Tomer, Yaron.

In: Clinical and Developmental Immunology, Vol. 10, No. 2-4, 06.2003, p. 161-165.

Research output: Contribution to journalArticle

Oppenheim, Yael ; Kim, Grace ; Ban, Yoshiyuki ; Unger, Pamela ; Concepcion, Erlinda ; Ando, Takao ; Tomer, Yaron. / The Effects of Alpha Interferon on the Development of Autoimmune Thyroiditis in the NOD H2h4 Mouse. In: Clinical and Developmental Immunology. 2003 ; Vol. 10, No. 2-4. pp. 161-165.
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