The effects of aerosolized dextran in a mouse model of Pseudomonas aeruginosa pulmonary infection

Ruth Bryan, Matthew Feldman, Sheryl C. Jawetz, Sujatha Rajan, Emily DiMango, H. Babette Tang, Lee Scheffler, David P. Speert, Alice Prince

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Airway infections initiated by the interaction of bacterial adhesins with carbohydrate receptors can be potentially prevented by nontoxic carbohydrate inhibitors. Intranasal inoculation of neonatal mice with Pseudomonas aeruginosa PAO1 caused pneumonia in 55% of control mice but in only 13% of mice inoculated 2 h after dextran inhalation (P <. 001) and in 28% inoculated 4 h after dextran inhalation (P = .02). PAO1 adherence to epithelial cells was inhibited by 50% in the presence of dextran. Dextran was well distributed throughout the airways and stimulated tumor necrosis factor- α production in murine lungs but not interleukin-8 production by human epithelial cell lines. Phagocytosis of PAO1 was not affected by dextran nor was killing by human neutrophils diminished. Administration of dextran by aerosol may prevent murine pneumonia by impeding bacterial access to epithelial receptors and by stimulation of the immune functions of the epithelium.

Original languageEnglish (US)
Pages (from-to)1449-1458
Number of pages10
JournalJournal of Infectious Diseases
Volume179
Issue number6
DOIs
StatePublished - Jun 7 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Bryan, R., Feldman, M., Jawetz, S. C., Rajan, S., DiMango, E., Tang, H. B., Scheffler, L., Speert, D. P., & Prince, A. (1999). The effects of aerosolized dextran in a mouse model of Pseudomonas aeruginosa pulmonary infection. Journal of Infectious Diseases, 179(6), 1449-1458. https://doi.org/10.1086/314755