The effects of aerosolized dextran in a mouse model of Pseudomonas aeruginosa pulmonary infection

Ruth Bryan, Matthew Feldman, Sheryl C. Jawetz, Sujatha Rajan, Emily DiMango, H. Babette Tang, Lee Scheffler, David P. Speert, Alice Prince

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Airway infections initiated by the interaction of bacterial adhesins with carbohydrate receptors can be potentially prevented by nontoxic carbohydrate inhibitors. Intranasal inoculation of neonatal mice with Pseudomonas aeruginosa PAO1 caused pneumonia in 55% of control mice but in only 13% of mice inoculated 2 h after dextran inhalation (P <. 001) and in 28% inoculated 4 h after dextran inhalation (P = .02). PAO1 adherence to epithelial cells was inhibited by 50% in the presence of dextran. Dextran was well distributed throughout the airways and stimulated tumor necrosis factor- α production in murine lungs but not interleukin-8 production by human epithelial cell lines. Phagocytosis of PAO1 was not affected by dextran nor was killing by human neutrophils diminished. Administration of dextran by aerosol may prevent murine pneumonia by impeding bacterial access to epithelial receptors and by stimulation of the immune functions of the epithelium.

Original languageEnglish (US)
Pages (from-to)1449-1458
Number of pages10
JournalJournal of Infectious Diseases
Volume179
Issue number6
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Dextrans
Pseudomonas aeruginosa
Lung
Infection
Inhalation
Bacterial Adhesins
Epithelial Cells
Carbohydrates
Bacterial Pneumonia
Aerosols
Interleukin-8
Phagocytosis
Pneumonia
Neutrophils
Epithelium
Tumor Necrosis Factor-alpha
Cell Line

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Bryan, R., Feldman, M., Jawetz, S. C., Rajan, S., DiMango, E., Tang, H. B., ... Prince, A. (1999). The effects of aerosolized dextran in a mouse model of Pseudomonas aeruginosa pulmonary infection. Journal of Infectious Diseases, 179(6), 1449-1458. https://doi.org/10.1086/314755

The effects of aerosolized dextran in a mouse model of Pseudomonas aeruginosa pulmonary infection. / Bryan, Ruth; Feldman, Matthew; Jawetz, Sheryl C.; Rajan, Sujatha; DiMango, Emily; Tang, H. Babette; Scheffler, Lee; Speert, David P.; Prince, Alice.

In: Journal of Infectious Diseases, Vol. 179, No. 6, 1999, p. 1449-1458.

Research output: Contribution to journalArticle

Bryan, R, Feldman, M, Jawetz, SC, Rajan, S, DiMango, E, Tang, HB, Scheffler, L, Speert, DP & Prince, A 1999, 'The effects of aerosolized dextran in a mouse model of Pseudomonas aeruginosa pulmonary infection', Journal of Infectious Diseases, vol. 179, no. 6, pp. 1449-1458. https://doi.org/10.1086/314755
Bryan, Ruth ; Feldman, Matthew ; Jawetz, Sheryl C. ; Rajan, Sujatha ; DiMango, Emily ; Tang, H. Babette ; Scheffler, Lee ; Speert, David P. ; Prince, Alice. / The effects of aerosolized dextran in a mouse model of Pseudomonas aeruginosa pulmonary infection. In: Journal of Infectious Diseases. 1999 ; Vol. 179, No. 6. pp. 1449-1458.
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