Abstract
To investigate the possible mechanisms by which interferon gamma (IFNγ) affects pregnancy, the expression of class II major histocompatibility complex (MHC) antigens and cleavage of DNA that is a hallmark of apoptosis in the placenta were examined by molecular biochemical techniques, and progesterone levels were examined by radioimmunoassay. The semi-quantitative analysis with reverse transcription-polymerase chain reaction showed that the expression of MHC class II antigen in placenta increased when rabbits were treated with high doses of IFNγ compared with the control. However, immunohistochemical study suggested that IFNγ did not affect MHC class II expression in trophoblasts, but had a stimulatory effect on its expression in maternal decidua and placental lymphocytes. DNA fragmentation analysis and terminal deoxynucleotidyl mediated-deoxyuridine triphosphate nick end labeling (TUNEL) assay indicated that the cleavage of DNA was detected in the placenta in both normal and IFNγ-treated pregnancy. Quantitative analysis of apoptotic cells revealed an increase in trophoblasts treated with IFNγ compared to those in normal pregnancy. Moreover, progesterone, which plays an important role in pregnancy, was reduced significantly in rabbits treated with IFNγ. The results suggested that IFNγ exerted its deleterious effect on pregnancy by inducing apoptosis in trophoblasts and by reducing the production of progesterone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 177-184 |
| Number of pages | 8 |
| Journal | Contraception |
| Volume | 65 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
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Keywords
- Apoptosis
- IFNγ
- MHC II
- Placenta
- Progesterone
- Rabbit
ASJC Scopus subject areas
- Medicine(all)
- Obstetrics and Gynecology
Cite this
The effect on MHC class II expression and apoptosis in placenta by IFNγ administration. / Liu, Zhe; Chen, Yun; Yang, Ying; Peng, Jing Pian.
In: Contraception, Vol. 65, No. 2, 2002, p. 177-184.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect on MHC class II expression and apoptosis in placenta by IFNγ administration
AU - Liu, Zhe
AU - Chen, Yun
AU - Yang, Ying
AU - Peng, Jing Pian
PY - 2002
Y1 - 2002
N2 - To investigate the possible mechanisms by which interferon gamma (IFNγ) affects pregnancy, the expression of class II major histocompatibility complex (MHC) antigens and cleavage of DNA that is a hallmark of apoptosis in the placenta were examined by molecular biochemical techniques, and progesterone levels were examined by radioimmunoassay. The semi-quantitative analysis with reverse transcription-polymerase chain reaction showed that the expression of MHC class II antigen in placenta increased when rabbits were treated with high doses of IFNγ compared with the control. However, immunohistochemical study suggested that IFNγ did not affect MHC class II expression in trophoblasts, but had a stimulatory effect on its expression in maternal decidua and placental lymphocytes. DNA fragmentation analysis and terminal deoxynucleotidyl mediated-deoxyuridine triphosphate nick end labeling (TUNEL) assay indicated that the cleavage of DNA was detected in the placenta in both normal and IFNγ-treated pregnancy. Quantitative analysis of apoptotic cells revealed an increase in trophoblasts treated with IFNγ compared to those in normal pregnancy. Moreover, progesterone, which plays an important role in pregnancy, was reduced significantly in rabbits treated with IFNγ. The results suggested that IFNγ exerted its deleterious effect on pregnancy by inducing apoptosis in trophoblasts and by reducing the production of progesterone.
AB - To investigate the possible mechanisms by which interferon gamma (IFNγ) affects pregnancy, the expression of class II major histocompatibility complex (MHC) antigens and cleavage of DNA that is a hallmark of apoptosis in the placenta were examined by molecular biochemical techniques, and progesterone levels were examined by radioimmunoassay. The semi-quantitative analysis with reverse transcription-polymerase chain reaction showed that the expression of MHC class II antigen in placenta increased when rabbits were treated with high doses of IFNγ compared with the control. However, immunohistochemical study suggested that IFNγ did not affect MHC class II expression in trophoblasts, but had a stimulatory effect on its expression in maternal decidua and placental lymphocytes. DNA fragmentation analysis and terminal deoxynucleotidyl mediated-deoxyuridine triphosphate nick end labeling (TUNEL) assay indicated that the cleavage of DNA was detected in the placenta in both normal and IFNγ-treated pregnancy. Quantitative analysis of apoptotic cells revealed an increase in trophoblasts treated with IFNγ compared to those in normal pregnancy. Moreover, progesterone, which plays an important role in pregnancy, was reduced significantly in rabbits treated with IFNγ. The results suggested that IFNγ exerted its deleterious effect on pregnancy by inducing apoptosis in trophoblasts and by reducing the production of progesterone.
KW - Apoptosis
KW - IFNγ
KW - MHC II
KW - Placenta
KW - Progesterone
KW - Rabbit
UR - http://www.scopus.com/inward/record.url?scp=0036208617&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036208617&partnerID=8YFLogxK
U2 - 10.1016/S0010-7824(01)00277-3
DO - 10.1016/S0010-7824(01)00277-3
M3 - Article
C2 - 11927122
AN - SCOPUS:0036208617
VL - 65
SP - 177
EP - 184
JO - Contraception
JF - Contraception
SN - 0010-7824
IS - 2
ER -