The effect of N-acetylglucosamine as a substrate for in vitro synthesis of glycosaminoglycans by human peritoneal mesothelial cells and fibroblasts.

A. Breborowicz, M. Kuzlan-Pawlaczyk, K. Wieczorowska-Tobis, J. Wisniewska, P. Tam, I. French, G. Wu

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The effect of N-acetylglucosamine (NAG) on in vitro synthesis of glycosaminoglycans by human peritoneal mesothelial cells and fibroblasts was studied. In contrast to isosmotic concentrations of glucose, NAG increases the synthesis of hyaluronan by mesothelial cells and fibroblasts in a dose-dependent manner. This effect of NAG can be demonstrated in the presence of increased glucose levels in a medium, or in a medium mixed with effluent dialysate obtained from continuous ambulatory peritoneal dialysis (CAPD) patients. Glucose inhibits synthesis of sulphated glycosaminoglycans by peritoneal mesothelial cells and fibroblasts, whereas NAG stimulates their production. Our results demonstrate that NAG is an effective stimulator of the in vitro glycosaminoglycans synthesis by human peritoneal mesothelial cells and fibroblasts.

Original languageEnglish (US)
Pages (from-to)31-35
Number of pages5
JournalAdvances in peritoneal dialysis. Conference on Peritoneal Dialysis
Volume14
StatePublished - 1998

ASJC Scopus subject areas

  • Medicine(all)

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