The effect of mercaptoethanol on flagellar morphogenesis in the amoeboflagellate Naegleria gruberi (Schardinger)

J. Wade, P. Satir

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

β-Mercaptoethanol added to cultures of Naegleria gruberi at any time after the induction of flagellation by washing, leveled the flagellation curve at a time 20 min later than the time of addition of the drug. When the mercaptoethanol was removed from blocked amoebae, there was subsequent recovery. Treatment with mercaptoethanol for 20-60 min caused excess delay in recovery such that the cells were setback to the time of induction (t0). Comparison of the time of sensitivity to mercaptoethanol vs. actinomycin D indicated that the primary effect of mercaptoethanol occurs at a later point in flagella morphogenesis than RNA synthesis. Sensitivity to actinomycin D characteristic of newly induced amoebae was not restored to such cells setback to t0 provided only a short time (< 120 min) had elapsed since induction. These results are interpreted in terms of mercaptoethanol's hypothesized effect on the assembly of precursor protein molecules into ordered structures and suggest that manufacture or polymerization of certain microtubular or fibrous elements begins almost immediately upon induction of flagellation.

Original languageEnglish (US)
Pages (from-to)81-92
Number of pages12
JournalExperimental Cell Research
Volume50
Issue number1
StatePublished - Apr 1968
Externally publishedYes

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Naegleria
Mercaptoethanol
Morphogenesis
Amoeba
Dactinomycin
Protein Precursors
Flagella
Polymerization
RNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cell Biology

Cite this

The effect of mercaptoethanol on flagellar morphogenesis in the amoeboflagellate Naegleria gruberi (Schardinger). / Wade, J.; Satir, P.

In: Experimental Cell Research, Vol. 50, No. 1, 04.1968, p. 81-92.

Research output: Contribution to journalArticle

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