The effect of HIV infection and HAART on inflammatory biomarkers in a population-based cohort of women

Sheila M. Keating, Elizabeth T. Golub, Marek Nowicki, Mary Young, Kathryn Anastos, Howard Crystal, Mardge H. Cohen, Jinbing Zhang, Ruth M. Greenblatt, Seema Desai, Shiquan Wu, Alan L. Landay, Stephen J. Gange, Philip J. Norris

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE: HIV causes inflammation that can be at least partially corrected by HAART. To determine the qualitative and quantitative nature of cytokine perturbation, we compared cytokine patterns in three HIV clinical groups, including HAART responders (HAART), untreated HIV noncontrollers, and HIV-uninfected (NEG). METHODS: Multiplex assays were used to measure 32 cytokines in a cross-sectional study of participants in the Women's Interagency HIV Study. Participants from three groups were included: HAART (n=17), noncontrollers (n=14), and HIV NEG (n=17). RESULTS: Several cytokines and chemokines showed significant differences between noncontrollers and NEG participants, including elevated interferon gamma-induced 10 (IP-10) and tumor necrosis factor-α (TNF-α) and decreased interleukin-12(p40) [IL-12(p40)], IL-15, and fibroblast growth factor-2 (FGF-2) in noncontroller participants. Biomarker levels among HAART women more closely resembled the NEG, with the exception of TNF-α and FGF-2. Secondary analyses of the combined HAART and noncontroller groups revealed that IP-10 showed a strong, positive correlation with viral load and negative correlation with CD4 + T-cell counts. The growth factors vascular endothelial growth factor, epidermal growth factor, and FGF-2 all showed a positive correlation with increased CD4 + T-cell counts. CONCLUSION: Untreated, progressive HIV infection was associated with decreased serum levels of cytokines important in T-cell homeostasis (IL-15) and T-cell phenotype determination (IL-12), and increased levels of innate inflammatory mediators such as IP-10 and TNF-α. HAART was associated with cytokine profiles that more closely resembled those of HIV-uninfected women. The distinctive pattern of cytokine levels in the three study groups may provide insights into HIV pathogenesis, and responses to therapy.

Original languageEnglish (US)
Pages (from-to)1823-1832
Number of pages10
JournalAIDS
Volume25
Issue number15
DOIs
StatePublished - Sep 24 2011

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Highly Active Antiretroviral Therapy
HIV Infections
Biomarkers
HIV
Cytokines
Population
Fibroblast Growth Factor 2
T-Lymphocytes
Interleukin-15
Tumor Necrosis Factor-alpha
Interleukin-12
CD4 Lymphocyte Count
Viral Load
Chemokines
Epidermal Growth Factor
Vascular Endothelial Growth Factor A
Interferon-gamma
Intercellular Signaling Peptides and Proteins
Homeostasis
Cross-Sectional Studies

Keywords

  • CD4 T cells
  • chemokines
  • cytokines
  • HAART
  • HIV

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Keating, S. M., Golub, E. T., Nowicki, M., Young, M., Anastos, K., Crystal, H., ... Norris, P. J. (2011). The effect of HIV infection and HAART on inflammatory biomarkers in a population-based cohort of women. AIDS, 25(15), 1823-1832. https://doi.org/10.1097/QAD.0b013e3283489d1f

The effect of HIV infection and HAART on inflammatory biomarkers in a population-based cohort of women. / Keating, Sheila M.; Golub, Elizabeth T.; Nowicki, Marek; Young, Mary; Anastos, Kathryn; Crystal, Howard; Cohen, Mardge H.; Zhang, Jinbing; Greenblatt, Ruth M.; Desai, Seema; Wu, Shiquan; Landay, Alan L.; Gange, Stephen J.; Norris, Philip J.

In: AIDS, Vol. 25, No. 15, 24.09.2011, p. 1823-1832.

Research output: Contribution to journalArticle

Keating, SM, Golub, ET, Nowicki, M, Young, M, Anastos, K, Crystal, H, Cohen, MH, Zhang, J, Greenblatt, RM, Desai, S, Wu, S, Landay, AL, Gange, SJ & Norris, PJ 2011, 'The effect of HIV infection and HAART on inflammatory biomarkers in a population-based cohort of women', AIDS, vol. 25, no. 15, pp. 1823-1832. https://doi.org/10.1097/QAD.0b013e3283489d1f
Keating, Sheila M. ; Golub, Elizabeth T. ; Nowicki, Marek ; Young, Mary ; Anastos, Kathryn ; Crystal, Howard ; Cohen, Mardge H. ; Zhang, Jinbing ; Greenblatt, Ruth M. ; Desai, Seema ; Wu, Shiquan ; Landay, Alan L. ; Gange, Stephen J. ; Norris, Philip J. / The effect of HIV infection and HAART on inflammatory biomarkers in a population-based cohort of women. In: AIDS. 2011 ; Vol. 25, No. 15. pp. 1823-1832.
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abstract = "OBJECTIVE: HIV causes inflammation that can be at least partially corrected by HAART. To determine the qualitative and quantitative nature of cytokine perturbation, we compared cytokine patterns in three HIV clinical groups, including HAART responders (HAART), untreated HIV noncontrollers, and HIV-uninfected (NEG). METHODS: Multiplex assays were used to measure 32 cytokines in a cross-sectional study of participants in the Women's Interagency HIV Study. Participants from three groups were included: HAART (n=17), noncontrollers (n=14), and HIV NEG (n=17). RESULTS: Several cytokines and chemokines showed significant differences between noncontrollers and NEG participants, including elevated interferon gamma-induced 10 (IP-10) and tumor necrosis factor-α (TNF-α) and decreased interleukin-12(p40) [IL-12(p40)], IL-15, and fibroblast growth factor-2 (FGF-2) in noncontroller participants. Biomarker levels among HAART women more closely resembled the NEG, with the exception of TNF-α and FGF-2. Secondary analyses of the combined HAART and noncontroller groups revealed that IP-10 showed a strong, positive correlation with viral load and negative correlation with CD4 + T-cell counts. The growth factors vascular endothelial growth factor, epidermal growth factor, and FGF-2 all showed a positive correlation with increased CD4 + T-cell counts. CONCLUSION: Untreated, progressive HIV infection was associated with decreased serum levels of cytokines important in T-cell homeostasis (IL-15) and T-cell phenotype determination (IL-12), and increased levels of innate inflammatory mediators such as IP-10 and TNF-α. HAART was associated with cytokine profiles that more closely resembled those of HIV-uninfected women. The distinctive pattern of cytokine levels in the three study groups may provide insights into HIV pathogenesis, and responses to therapy.",
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AU - Crystal, Howard

AU - Cohen, Mardge H.

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