The effect of epidermal growth factor on the septic complications of acute pancreatitis

Qiang Liu, Goldie Djuricin, Catherine Nathan, Paolo Gattuso, Robert A. Weinstein, Richard A. Prinz

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Bacterial translocation (BT) from the gastrointestinal tract to mesenteric lymph nodes (MLN) and other extraintestinal organs is an important source of infection in acute pancreatitis (AP). Epidermal growth factor (EGF), a peptide hormone with trophic effects on gut mucosa, has decreased intestinal mucosal injury in septic rats and decreased burn-induced BT in mice. The purpose of this study is to examine whether EGF could affect BT in acute necrotizing pancreatitis. Forty-eight male Sprague-Dawley rats (250- 350 g) were studied. AP was induced in Group I and Group II by pressure injection of 3% taurocholate and trypsin into the biliopancreatic duct (1 ml/kg of body weight). Group III and Group IV underwent laparotomy without induction of acute pancreatitis. Group I rats received human recombinant EGF (100 μg/kg, subcutaneously twice daily) and Group II rats received a similar volume of 0.1% bovine serum albumin as a placebo postoperatively. Group III and Group IV received EGF and placebo, respectively. At 48 hr postoperatively, blood was drawn for culture and amylase determinations. Jejunum and ileum were obtained to measure mucosal protein content, mucosal thickness, villus height, and crypt depth. Specimens from MLN, spleen, liver, pancreas, and cecum were harvested for pathology and culture of gram positive (G+), gram negative (G-), and anaerobic bacteria. Heal mucosal protein levels were increased significantly in Group I (1.96 ± 0.14 mg/cm) compared to Group II (0.95 ± 0.15 mg/cm intestinal segment) (P < 0.01). Jejunal and ileal mucosal thickness, villus height, and crypt depth in Group I were significantly increased when compared to Group II (P < 0.05). All 12 rats in Group II had BT to MLN compared to 58% (7 of 12 rats) in Group I (P < 0.05). Thirty-three percent (4 of 12 rats) had BT to distant sites such as pancreas, spleen, liver, and/or blood in Group I vs 83% (10 of 12 rats) in Group II (P < 0.05). EGF treatment minimizes intestinal damage, decreases BT to MLN and bacterial spread to distant sites, and may be beneficial in preventing septic complications in AP.

Original languageEnglish (US)
Pages (from-to)171-177
Number of pages7
JournalJournal of Surgical Research
Volume69
Issue number1
DOIs
StatePublished - Apr 1997
Externally publishedYes

Fingerprint

Bacterial Translocation
Epidermal Growth Factor
Pancreatitis
Lymph Nodes
Pancreas
Spleen
Gram-Negative Anaerobic Bacteria
Placebos
Acute Necrotizing Pancreatitis
Taurocholic Acid
Cecum
Peptide Hormones
Liver
Jejunum
Amylases
Blood Group Antigens
Bovine Serum Albumin
Burns
Ileum
Laparotomy

ASJC Scopus subject areas

  • Surgery

Cite this

The effect of epidermal growth factor on the septic complications of acute pancreatitis. / Liu, Qiang; Djuricin, Goldie; Nathan, Catherine; Gattuso, Paolo; Weinstein, Robert A.; Prinz, Richard A.

In: Journal of Surgical Research, Vol. 69, No. 1, 04.1997, p. 171-177.

Research output: Contribution to journalArticle

Liu, Qiang ; Djuricin, Goldie ; Nathan, Catherine ; Gattuso, Paolo ; Weinstein, Robert A. ; Prinz, Richard A. / The effect of epidermal growth factor on the septic complications of acute pancreatitis. In: Journal of Surgical Research. 1997 ; Vol. 69, No. 1. pp. 171-177.
@article{944b7265a26f4b618b82ca29532c37ff,
title = "The effect of epidermal growth factor on the septic complications of acute pancreatitis",
abstract = "Bacterial translocation (BT) from the gastrointestinal tract to mesenteric lymph nodes (MLN) and other extraintestinal organs is an important source of infection in acute pancreatitis (AP). Epidermal growth factor (EGF), a peptide hormone with trophic effects on gut mucosa, has decreased intestinal mucosal injury in septic rats and decreased burn-induced BT in mice. The purpose of this study is to examine whether EGF could affect BT in acute necrotizing pancreatitis. Forty-eight male Sprague-Dawley rats (250- 350 g) were studied. AP was induced in Group I and Group II by pressure injection of 3{\%} taurocholate and trypsin into the biliopancreatic duct (1 ml/kg of body weight). Group III and Group IV underwent laparotomy without induction of acute pancreatitis. Group I rats received human recombinant EGF (100 μg/kg, subcutaneously twice daily) and Group II rats received a similar volume of 0.1{\%} bovine serum albumin as a placebo postoperatively. Group III and Group IV received EGF and placebo, respectively. At 48 hr postoperatively, blood was drawn for culture and amylase determinations. Jejunum and ileum were obtained to measure mucosal protein content, mucosal thickness, villus height, and crypt depth. Specimens from MLN, spleen, liver, pancreas, and cecum were harvested for pathology and culture of gram positive (G+), gram negative (G-), and anaerobic bacteria. Heal mucosal protein levels were increased significantly in Group I (1.96 ± 0.14 mg/cm) compared to Group II (0.95 ± 0.15 mg/cm intestinal segment) (P < 0.01). Jejunal and ileal mucosal thickness, villus height, and crypt depth in Group I were significantly increased when compared to Group II (P < 0.05). All 12 rats in Group II had BT to MLN compared to 58{\%} (7 of 12 rats) in Group I (P < 0.05). Thirty-three percent (4 of 12 rats) had BT to distant sites such as pancreas, spleen, liver, and/or blood in Group I vs 83{\%} (10 of 12 rats) in Group II (P < 0.05). EGF treatment minimizes intestinal damage, decreases BT to MLN and bacterial spread to distant sites, and may be beneficial in preventing septic complications in AP.",
author = "Qiang Liu and Goldie Djuricin and Catherine Nathan and Paolo Gattuso and Weinstein, {Robert A.} and Prinz, {Richard A.}",
year = "1997",
month = "4",
doi = "10.1006/jsre.1997.5069",
language = "English (US)",
volume = "69",
pages = "171--177",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The effect of epidermal growth factor on the septic complications of acute pancreatitis

AU - Liu, Qiang

AU - Djuricin, Goldie

AU - Nathan, Catherine

AU - Gattuso, Paolo

AU - Weinstein, Robert A.

AU - Prinz, Richard A.

PY - 1997/4

Y1 - 1997/4

N2 - Bacterial translocation (BT) from the gastrointestinal tract to mesenteric lymph nodes (MLN) and other extraintestinal organs is an important source of infection in acute pancreatitis (AP). Epidermal growth factor (EGF), a peptide hormone with trophic effects on gut mucosa, has decreased intestinal mucosal injury in septic rats and decreased burn-induced BT in mice. The purpose of this study is to examine whether EGF could affect BT in acute necrotizing pancreatitis. Forty-eight male Sprague-Dawley rats (250- 350 g) were studied. AP was induced in Group I and Group II by pressure injection of 3% taurocholate and trypsin into the biliopancreatic duct (1 ml/kg of body weight). Group III and Group IV underwent laparotomy without induction of acute pancreatitis. Group I rats received human recombinant EGF (100 μg/kg, subcutaneously twice daily) and Group II rats received a similar volume of 0.1% bovine serum albumin as a placebo postoperatively. Group III and Group IV received EGF and placebo, respectively. At 48 hr postoperatively, blood was drawn for culture and amylase determinations. Jejunum and ileum were obtained to measure mucosal protein content, mucosal thickness, villus height, and crypt depth. Specimens from MLN, spleen, liver, pancreas, and cecum were harvested for pathology and culture of gram positive (G+), gram negative (G-), and anaerobic bacteria. Heal mucosal protein levels were increased significantly in Group I (1.96 ± 0.14 mg/cm) compared to Group II (0.95 ± 0.15 mg/cm intestinal segment) (P < 0.01). Jejunal and ileal mucosal thickness, villus height, and crypt depth in Group I were significantly increased when compared to Group II (P < 0.05). All 12 rats in Group II had BT to MLN compared to 58% (7 of 12 rats) in Group I (P < 0.05). Thirty-three percent (4 of 12 rats) had BT to distant sites such as pancreas, spleen, liver, and/or blood in Group I vs 83% (10 of 12 rats) in Group II (P < 0.05). EGF treatment minimizes intestinal damage, decreases BT to MLN and bacterial spread to distant sites, and may be beneficial in preventing septic complications in AP.

AB - Bacterial translocation (BT) from the gastrointestinal tract to mesenteric lymph nodes (MLN) and other extraintestinal organs is an important source of infection in acute pancreatitis (AP). Epidermal growth factor (EGF), a peptide hormone with trophic effects on gut mucosa, has decreased intestinal mucosal injury in septic rats and decreased burn-induced BT in mice. The purpose of this study is to examine whether EGF could affect BT in acute necrotizing pancreatitis. Forty-eight male Sprague-Dawley rats (250- 350 g) were studied. AP was induced in Group I and Group II by pressure injection of 3% taurocholate and trypsin into the biliopancreatic duct (1 ml/kg of body weight). Group III and Group IV underwent laparotomy without induction of acute pancreatitis. Group I rats received human recombinant EGF (100 μg/kg, subcutaneously twice daily) and Group II rats received a similar volume of 0.1% bovine serum albumin as a placebo postoperatively. Group III and Group IV received EGF and placebo, respectively. At 48 hr postoperatively, blood was drawn for culture and amylase determinations. Jejunum and ileum were obtained to measure mucosal protein content, mucosal thickness, villus height, and crypt depth. Specimens from MLN, spleen, liver, pancreas, and cecum were harvested for pathology and culture of gram positive (G+), gram negative (G-), and anaerobic bacteria. Heal mucosal protein levels were increased significantly in Group I (1.96 ± 0.14 mg/cm) compared to Group II (0.95 ± 0.15 mg/cm intestinal segment) (P < 0.01). Jejunal and ileal mucosal thickness, villus height, and crypt depth in Group I were significantly increased when compared to Group II (P < 0.05). All 12 rats in Group II had BT to MLN compared to 58% (7 of 12 rats) in Group I (P < 0.05). Thirty-three percent (4 of 12 rats) had BT to distant sites such as pancreas, spleen, liver, and/or blood in Group I vs 83% (10 of 12 rats) in Group II (P < 0.05). EGF treatment minimizes intestinal damage, decreases BT to MLN and bacterial spread to distant sites, and may be beneficial in preventing septic complications in AP.

UR - http://www.scopus.com/inward/record.url?scp=0031127991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031127991&partnerID=8YFLogxK

U2 - 10.1006/jsre.1997.5069

DO - 10.1006/jsre.1997.5069

M3 - Article

C2 - 9202665

AN - SCOPUS:0031127991

VL - 69

SP - 171

EP - 177

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 1

ER -