The effect of divalent cations and core polymerase in the abortive initiation by escherichia coli dna-dependent rna polymerase using phosphonate dinucleotide primers

Ales Cvekl, Květa Horská, Karel Šebesta, Ivan Rosenberg, Antonín Holý

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of divalent Mg2+ and Mn2+ cations on the elongation of ApU, UpA and their 3'O- and 5'O-phosphonylmethyl analogues by RNA polymerase holoenzyme to the corresponding trinucleo-tides on a poly(dA-dT) template was investigated. In contrast to Mgz+ ions, Mn2+ ions enhance abortive trinucleotide synthesis. This effect is more pronounced with phosphonylmethyl analogues. The core enzyme cannot catalyze the elongation of either (2'5' UpA or phosphonylmethyl analogues. The localization of the divalent cation activator, as well as the role of the σ subunit at the catalytic centre of the holoenzyme, is discussed.

Original languageEnglish (US)
Pages (from-to)275-281
Number of pages7
JournalBiocatalysis and Biotransformation
Volume1
Issue number4
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Organophosphonates
Holoenzymes
Divalent Cations
DNA-Directed RNA Polymerases
Escherichia coli
Elongation
Positive ions
Poly dA-dT
Ions
Tides
RNA
Cations
Catalytic Domain
Enzymes
uridylyl-(3'-5')-adenosine

Keywords

  • Core polymerase
  • Divalent cations
  • Phosphonate dinucleotide primers

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Catalysis

Cite this

The effect of divalent cations and core polymerase in the abortive initiation by escherichia coli dna-dependent rna polymerase using phosphonate dinucleotide primers. / Cvekl, Ales; Horská, Květa; Šebesta, Karel; Rosenberg, Ivan; Holý, Antonín.

In: Biocatalysis and Biotransformation, Vol. 1, No. 4, 1988, p. 275-281.

Research output: Contribution to journalArticle

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AU - Rosenberg, Ivan

AU - Holý, Antonín

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