The effect of divalent cations and core polymerase in the abortive initiation by escherichia coli dna-dependent rna polymerase using phosphonate dinucleotide primers

Ales Cvekl, Květa Horská, Karel Šebesta, Ivan Rosenberg, Antonín Holý

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The effect of divalent Mg2+ and Mn2+ cations on the elongation of ApU, UpA and their 3'O- and 5'O-phosphonylmethyl analogues by RNA polymerase holoenzyme to the corresponding trinucleo-tides on a poly(dA-dT) template was investigated. In contrast to Mgz+ ions, Mn2+ ions enhance abortive trinucleotide synthesis. This effect is more pronounced with phosphonylmethyl analogues. The core enzyme cannot catalyze the elongation of either (2'5' UpA or phosphonylmethyl analogues. The localization of the divalent cation activator, as well as the role of the σ subunit at the catalytic centre of the holoenzyme, is discussed.

Original languageEnglish (US)
Pages (from-to)275-281
Number of pages7
JournalBiocatalysis and Biotransformation
Volume1
Issue number4
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

Keywords

  • Core polymerase
  • Divalent cations
  • Phosphonate dinucleotide primers

ASJC Scopus subject areas

  • Biotechnology
  • Catalysis
  • Biochemistry

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