The effect of bone morphogenetic protein-2 on osteosarcoma metastasis

Jonathan Gill, Patrick Connolly, Michael Roth, So Hak Chung, Wendong Zhang, Sajida Piperdi, Bang H. Hoang, Rui Yang, Hillary Guzik, Jonathan Morris, Richard Gorlick, David S. Geller

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4 Scopus citations

Abstract

Purpose Bone Morphogenetic Protein-2 (BMP-2) may offer the potential to enhance allograft-host osseous union in limb-salvage surgery following osteosarcoma resection. However, there is concern regarding the effect of locally applied BMP-2 on tumor recurrence and metastasis. The purpose of this project was to evaluate the effect of exogenous BMP-2 on osteosarcoma migration and invasion across a panel of tumor cell lines in vitro and to characterize the effect of BMP-2 on pulmonary osteosarcoma metastasis within a xenograft model. Experimental design The effect of BMP-2 on in vitro tumor growth and development was assessed across multiple standard and patient-derived xenograft osteosarcoma cell lines. Tumor migration capacity, invasion, and cell proliferation were characterized. In addition, the effect on metastasis was measured using a xenograft model following tail-vein injection. The effect of exogenous BMP-2 on the development of metastases was measured following both single and multiple BMP-2 administrations. Results There was no significant difference in migration capacity, invasion, or cell proliferation between the BMP-2 treated and the untreated osteosarcoma cell lines. There was no significant difference in pulmonary metastases between either the single-dose or multi-dose BMP-2 treated animals and the untreated control animals. Conclusions In the model systems tested, the addition of BMP-2 does not increase osteosarcoma proliferation, migration, invasion, or metastasis to the lungs.

Original languageEnglish (US)
Article numbere0173322
JournalPLoS One
Volume12
Issue number3
DOIs
StatePublished - Mar 1 2017

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ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Gill, J., Connolly, P., Roth, M., Chung, S. H., Zhang, W., Piperdi, S., Hoang, B. H., Yang, R., Guzik, H., Morris, J., Gorlick, R., & Geller, D. S. (2017). The effect of bone morphogenetic protein-2 on osteosarcoma metastasis. PLoS One, 12(3), [e0173322]. https://doi.org/10.1371/journal.pone.0173322