The effect of bone morphogenetic protein-2 on osteosarcoma metastasis

Jonathan Gill, Patrick Connolly, Michael Roth, So Hak Chung, Wendong Zhang, Sajida Piperdi, Bang H. Hoang, Rui Yang, Hillary Guzik, Jonathan Morris, Richard Gorlick, David S. Geller

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose Bone Morphogenetic Protein-2 (BMP-2) may offer the potential to enhance allograft-host osseous union in limb-salvage surgery following osteosarcoma resection. However, there is concern regarding the effect of locally applied BMP-2 on tumor recurrence and metastasis. The purpose of this project was to evaluate the effect of exogenous BMP-2 on osteosarcoma migration and invasion across a panel of tumor cell lines in vitro and to characterize the effect of BMP-2 on pulmonary osteosarcoma metastasis within a xenograft model. Experimental design The effect of BMP-2 on in vitro tumor growth and development was assessed across multiple standard and patient-derived xenograft osteosarcoma cell lines. Tumor migration capacity, invasion, and cell proliferation were characterized. In addition, the effect on metastasis was measured using a xenograft model following tail-vein injection. The effect of exogenous BMP-2 on the development of metastases was measured following both single and multiple BMP-2 administrations. Results There was no significant difference in migration capacity, invasion, or cell proliferation between the BMP-2 treated and the untreated osteosarcoma cell lines. There was no significant difference in pulmonary metastases between either the single-dose or multi-dose BMP-2 treated animals and the untreated control animals. Conclusions In the model systems tested, the addition of BMP-2 does not increase osteosarcoma proliferation, migration, invasion, or metastasis to the lungs.

Original languageEnglish (US)
Article numbere0173322
JournalPLoS One
Volume12
Issue number3
DOIs
StatePublished - Mar 1 2017

Fingerprint

bone morphogenetic proteins
Bone Morphogenetic Protein 2
osteosarcoma
Osteosarcoma
metastasis
Neoplasm Metastasis
Tumors
Heterografts
lungs
Cells
cell lines
Cell proliferation
Lung
neoplasms
cell proliferation
Animals
Cell Proliferation
Salvaging
Cell Line
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Gill, J., Connolly, P., Roth, M., Chung, S. H., Zhang, W., Piperdi, S., ... Geller, D. S. (2017). The effect of bone morphogenetic protein-2 on osteosarcoma metastasis. PLoS One, 12(3), [e0173322]. https://doi.org/10.1371/journal.pone.0173322

The effect of bone morphogenetic protein-2 on osteosarcoma metastasis. / Gill, Jonathan; Connolly, Patrick; Roth, Michael; Chung, So Hak; Zhang, Wendong; Piperdi, Sajida; Hoang, Bang H.; Yang, Rui; Guzik, Hillary; Morris, Jonathan; Gorlick, Richard; Geller, David S.

In: PLoS One, Vol. 12, No. 3, e0173322, 01.03.2017.

Research output: Contribution to journalArticle

Gill, J, Connolly, P, Roth, M, Chung, SH, Zhang, W, Piperdi, S, Hoang, BH, Yang, R, Guzik, H, Morris, J, Gorlick, R & Geller, DS 2017, 'The effect of bone morphogenetic protein-2 on osteosarcoma metastasis', PLoS One, vol. 12, no. 3, e0173322. https://doi.org/10.1371/journal.pone.0173322
Gill J, Connolly P, Roth M, Chung SH, Zhang W, Piperdi S et al. The effect of bone morphogenetic protein-2 on osteosarcoma metastasis. PLoS One. 2017 Mar 1;12(3). e0173322. https://doi.org/10.1371/journal.pone.0173322
Gill, Jonathan ; Connolly, Patrick ; Roth, Michael ; Chung, So Hak ; Zhang, Wendong ; Piperdi, Sajida ; Hoang, Bang H. ; Yang, Rui ; Guzik, Hillary ; Morris, Jonathan ; Gorlick, Richard ; Geller, David S. / The effect of bone morphogenetic protein-2 on osteosarcoma metastasis. In: PLoS One. 2017 ; Vol. 12, No. 3.
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abstract = "Purpose Bone Morphogenetic Protein-2 (BMP-2) may offer the potential to enhance allograft-host osseous union in limb-salvage surgery following osteosarcoma resection. However, there is concern regarding the effect of locally applied BMP-2 on tumor recurrence and metastasis. The purpose of this project was to evaluate the effect of exogenous BMP-2 on osteosarcoma migration and invasion across a panel of tumor cell lines in vitro and to characterize the effect of BMP-2 on pulmonary osteosarcoma metastasis within a xenograft model. Experimental design The effect of BMP-2 on in vitro tumor growth and development was assessed across multiple standard and patient-derived xenograft osteosarcoma cell lines. Tumor migration capacity, invasion, and cell proliferation were characterized. In addition, the effect on metastasis was measured using a xenograft model following tail-vein injection. The effect of exogenous BMP-2 on the development of metastases was measured following both single and multiple BMP-2 administrations. Results There was no significant difference in migration capacity, invasion, or cell proliferation between the BMP-2 treated and the untreated osteosarcoma cell lines. There was no significant difference in pulmonary metastases between either the single-dose or multi-dose BMP-2 treated animals and the untreated control animals. Conclusions In the model systems tested, the addition of BMP-2 does not increase osteosarcoma proliferation, migration, invasion, or metastasis to the lungs.",
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