The dynamics of mammalian P body transport, assembly, and disassembly in vivo

Adva Aizer, Yehuda Brody, Lian Wee Ler, Nahum Sonenberg, Robert H. Singer, Yaron Shav-Tal

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Exported mRNAs are targeted for translation or can undergo degradation by several decay mechanisms. The 5′→3′ degradation machinery localizes to cytoplasmic P bodies (PBs). We followed the dynamic properties of PBs in vivo and investigated the mechanism by which PBs scan the cytoplasm. Using proteins of the decapping machinery, we asked whether PBs actively scan the cytoplasm or whether a diffusion-based mechanism is sufficient. Live-cell imaging showed that PBs were anchored mainly to microtubules. Quantitative single-particle tracking demonstrated that most PBs exhibited spatially confined motion dependent on microtubule motion, whereas stationary PB pairs were identified at the centrosome. Some PBs translocated in long-range movements on microtubules. PB mobility was compared with mitochondria, endoplasmic reticulum, peroxisomes, SMN bodies, and stress granules, and diffusion coefficients were calculated. Disruption of the microtubule network caused a significant reduction in PB mobility together with an induction of PB assembly. However, FRAP measurements showed that the dynamic flux of assembled PB components was not affected by such treatments. FRAP analysis showed that the decapping enzyme Dcp2 is a nondynamic PB core protein, whereas Dcp1 proteins continuously exchanged with the cytoplasm. This study reveals the mechanism of PB transport, and it demonstrates how PB assembly and disassembly integrate with the presence of an intact cytoskeleton.

Original languageEnglish (US)
Pages (from-to)4154-4166
Number of pages13
JournalMolecular Biology of the Cell
Volume19
Issue number10
DOIs
StatePublished - Oct 2008

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Microtubules
Cytoplasm
Centrosome
Proteins
Peroxisomes
Protein Biosynthesis
Cytoskeleton
Endoplasmic Reticulum
Mitochondria

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The dynamics of mammalian P body transport, assembly, and disassembly in vivo. / Aizer, Adva; Brody, Yehuda; Ler, Lian Wee; Sonenberg, Nahum; Singer, Robert H.; Shav-Tal, Yaron.

In: Molecular Biology of the Cell, Vol. 19, No. 10, 10.2008, p. 4154-4166.

Research output: Contribution to journalArticle

Aizer, Adva ; Brody, Yehuda ; Ler, Lian Wee ; Sonenberg, Nahum ; Singer, Robert H. ; Shav-Tal, Yaron. / The dynamics of mammalian P body transport, assembly, and disassembly in vivo. In: Molecular Biology of the Cell. 2008 ; Vol. 19, No. 10. pp. 4154-4166.
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