The dynamics of mammalian P body transport, assembly, and disassembly in vivo

A. Aizer, Y. Brody, L. W. Ler, N. Sonenberg, Robert H. Singer, Y. Shav-Tal

Research output: Contribution to journalArticle

Abstract

The degradation of mRNA in eukaryotes begins with deadenylation, followed by degradation through one of two pathways: 3' → 5' cleavage by the exosome complex or 5' → 3' cleavage which begins with the removal of the 5'-7meG cap. The second mechanism occurs in cytoplasmic foci known as P bodies and involves multiple factors, such as decapping proteins and nucleases. This study examines the movement and interactions of P bodies (PBs) in the cytoplasm. PB movement was tracked by fluorescence microscopy and live cell imaging, using fluorescent proteins fused to conserved PB components such as decapping proteins. PBs were mostly found in the inner cytoplasmic region or near the nuclear envelope (Fig. 1), and, although dynamic, exhibited a tendency to remain in the same spatial neighborhood throughout the cell cycle. These confined movements suggest association with filamentous networks. PBs were shown to travel on microtubule networks and were also found to be associated with actin and centrosomes. When microtubules were disrupted by addition of inhibitors such as nocoda-zole and vinblastine, PB mobility was significantly impaired. This suggests that the microtubule network is used by PB bodies for anchoring and moving within the cytoplasm.

Original languageEnglish (US)
Pages (from-to)395-396
Number of pages2
JournalChemtracts
Volume21
Issue number10
StatePublished - Oct 2008
Externally publishedYes

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Microtubules
Cytoplasm
Exosomes
Degradation
Centrosome
Proteins
Vinblastine
Fluorescence microscopy
Nuclear Envelope
RNA Stability
Eukaryota
Fluorescence Microscopy
Actins
Cell Cycle
Cells
Association reactions
Imaging techniques
Messenger RNA

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Molecular Biology

Cite this

Aizer, A., Brody, Y., Ler, L. W., Sonenberg, N., Singer, R. H., & Shav-Tal, Y. (2008). The dynamics of mammalian P body transport, assembly, and disassembly in vivo. Chemtracts, 21(10), 395-396.

The dynamics of mammalian P body transport, assembly, and disassembly in vivo. / Aizer, A.; Brody, Y.; Ler, L. W.; Sonenberg, N.; Singer, Robert H.; Shav-Tal, Y.

In: Chemtracts, Vol. 21, No. 10, 10.2008, p. 395-396.

Research output: Contribution to journalArticle

Aizer, A, Brody, Y, Ler, LW, Sonenberg, N, Singer, RH & Shav-Tal, Y 2008, 'The dynamics of mammalian P body transport, assembly, and disassembly in vivo', Chemtracts, vol. 21, no. 10, pp. 395-396.
Aizer A, Brody Y, Ler LW, Sonenberg N, Singer RH, Shav-Tal Y. The dynamics of mammalian P body transport, assembly, and disassembly in vivo. Chemtracts. 2008 Oct;21(10):395-396.
Aizer, A. ; Brody, Y. ; Ler, L. W. ; Sonenberg, N. ; Singer, Robert H. ; Shav-Tal, Y. / The dynamics of mammalian P body transport, assembly, and disassembly in vivo. In: Chemtracts. 2008 ; Vol. 21, No. 10. pp. 395-396.
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