The Drosophila snr1 and brm proteins are related to yeast SWI/SNF proteins and are components of a large protein complex

A. K. Dingwall, S. J. Beek, C. M. McCallum, J. W. Tamkun, Ganjam V. Kalpana, S. P. Goff, M. P. Scott

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

During most of Drosophila development the regulation of homeotic gene transcription is controlled by two groups of regulatory genes, the trithorax group of activators and the Polycomb group of repressors. brahma (brm), a member of the trithorax group, encodes a protein related to the yeast SWI2/SNF2 protein, a subunit of a protein complex that assists sequence- specific activator proteins by alleviating the repressive effects of chromatin. To learn more about the molecular mechanisms underlying the regulation of homeotic gene transcription, we have investigated whether a similar complex exists in flies. We identified the Drosophila snr1 gene, a potential homologue of the yeast SNF5 gene that encodes a subunit of the yeast SWI/SNF complex. The snr1 gene is essential and genetically interacts with brm and trithorax (trx), suggesting cooperation in regulating homeotic gene transcription. The spatial and temporal patterns of expression of snr1 are similar to those of brm. The snr1 and brm proteins are present in a large (>2 x 106 Da) complex, and they co-immunoprecipitate from Drosophila extracts. These findings provide direct evidence for conservation of the SWI/SNF complex in higher eucaryotes and suggest that the Drosophila brm/snr1 complex plays an important role in maintaining homeotic gene transcription during development by counteracting the repressive effects of chromatin.

Original languageEnglish (US)
Pages (from-to)777-791
Number of pages15
JournalMolecular Biology of the Cell
Volume6
Issue number7
StatePublished - 1995
Externally publishedYes

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Homeobox Genes
Drosophila
Yeasts
Chromatin
Proteins
Essential Genes
Protein Subunits
Regulator Genes
Diptera
Genes
Drosophila brm protein
Drosophila Snr1 protein

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Dingwall, A. K., Beek, S. J., McCallum, C. M., Tamkun, J. W., Kalpana, G. V., Goff, S. P., & Scott, M. P. (1995). The Drosophila snr1 and brm proteins are related to yeast SWI/SNF proteins and are components of a large protein complex. Molecular Biology of the Cell, 6(7), 777-791.

The Drosophila snr1 and brm proteins are related to yeast SWI/SNF proteins and are components of a large protein complex. / Dingwall, A. K.; Beek, S. J.; McCallum, C. M.; Tamkun, J. W.; Kalpana, Ganjam V.; Goff, S. P.; Scott, M. P.

In: Molecular Biology of the Cell, Vol. 6, No. 7, 1995, p. 777-791.

Research output: Contribution to journalArticle

Dingwall, AK, Beek, SJ, McCallum, CM, Tamkun, JW, Kalpana, GV, Goff, SP & Scott, MP 1995, 'The Drosophila snr1 and brm proteins are related to yeast SWI/SNF proteins and are components of a large protein complex', Molecular Biology of the Cell, vol. 6, no. 7, pp. 777-791.
Dingwall, A. K. ; Beek, S. J. ; McCallum, C. M. ; Tamkun, J. W. ; Kalpana, Ganjam V. ; Goff, S. P. ; Scott, M. P. / The Drosophila snr1 and brm proteins are related to yeast SWI/SNF proteins and are components of a large protein complex. In: Molecular Biology of the Cell. 1995 ; Vol. 6, No. 7. pp. 777-791.
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