The drosophila kinesin-13, KLP59D, impacts Pacman- and flux-based chromosome movement

Uttama Rath, Gregory C. Rogers, Dongyan Tan, Maria Ana Gomez-Ferreria, Daniel W. Buster, Hernando J. Sosa, David J. Sharp

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Chromosome movements are linked to the active depolymerization of spindle microtubule (MT) ends. Here we identify the kinesin-13 family member, KLP59D, as a novel and uniquely important regulator of spindle MT dynamics and chromosome motility in Drosophila somatic cells. During prometaphase and metaphase, depletion of KLP59D, which targets to centrosomes and outer kinetochores, suppresses the depolymerization of spindle pole - associated MT minus ends, thereby inhibiting poleward tubulin Flux. Subsequently, during anaphase, loss of KLP59D strongly attenuates chromatid-to-pole motion by suppressing the depolymerization of both minus and plus ends of kinetochore-associated MTs. The mechanism of KLP59D's impact on spindle MT plus and minus ends appears to differ. Our data support a model in which KLP59D directly depolymerizes kinetochore-associated plus ends during anaphase, but influences minus ends indirectly by localizing the pole-associated MT depolymerase KLP10A. Finally, electron microscopy indicates that, unlike the other Drosophila kinesin-13s, KLP59D is largely incapable of oligomerizing into MT-associated rings in vitro, suggesting that such structures are not a requisite feature of kinetochore-based MT disassembly and chromosome movements.

Original languageEnglish (US)
Pages (from-to)4696-4705
Number of pages10
JournalMolecular Biology of the Cell
Volume20
Issue number22
DOIs
StatePublished - 2009

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Kinesin
Microtubules
Drosophila
Chromosomes
Kinetochores
Anaphase
Prometaphase
Spindle Poles
Centrosome
Chromatids
Tubulin
Metaphase
Electron Microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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The drosophila kinesin-13, KLP59D, impacts Pacman- and flux-based chromosome movement. / Rath, Uttama; Rogers, Gregory C.; Tan, Dongyan; Gomez-Ferreria, Maria Ana; Buster, Daniel W.; Sosa, Hernando J.; Sharp, David J.

In: Molecular Biology of the Cell, Vol. 20, No. 22, 2009, p. 4696-4705.

Research output: Contribution to journalArticle

Rath, Uttama ; Rogers, Gregory C. ; Tan, Dongyan ; Gomez-Ferreria, Maria Ana ; Buster, Daniel W. ; Sosa, Hernando J. ; Sharp, David J. / The drosophila kinesin-13, KLP59D, impacts Pacman- and flux-based chromosome movement. In: Molecular Biology of the Cell. 2009 ; Vol. 20, No. 22. pp. 4696-4705.
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