The DNA sequence of the sulfate activation locus from Escherichia coli K-12

Thomas S. Leyh, Thomas F. Vogt, Ya Suo

Research output: Contribution to journalArticle

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Abstract

The DNA sequence of the sulfate activation locus from Escherichia coli K-12 has been determined. The sequence includes the structural genes encoding the enzymes ATP sulfurylase (cysD and cysN) and APS kinase (cysC) which catalyze the synthesis of activated sulfate. These are the only genes known to reside in the sulfate activation operon. Consensus elements of the operon promoter were identified, and the start codons and open reading frames of the Cys polypeptides were determined. During this work, another gene, iap, was partially sequenced and mapped. The activity of ATP sulfurylase is stimulated by an intrinsic GTPase. Comparison of the primary sequences of CysN and Ef-Tu revealed that CysN has conserved many of the residues integral to the three-dimensional structure important for guanine nucleotide binding in Ef-Tu and RAS. nodP and nodQ, from Rhizobium meliloti, are essential for nodulation in leguminous plants. The Cys and Nod proteins are remarkably similar. NodP appears to be the smaller subunit of ATP sulfurylase. NodQ encodes homologues of both CysN and CysC; thus, these enzymes may be covalently associated in R. meliloti. The consensus GTP-binding sequences of NodQ and CysN are identical suggesting that NodQ encodes a regulatory GTPase.

Original languageEnglish (US)
Pages (from-to)10405-10410
Number of pages6
JournalJournal of Biological Chemistry
Volume267
Issue number15
StatePublished - May 25 1992

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Sulfate Adenylyltransferase
DNA sequences
Escherichia coli
Sulfates
Sinorhizobium meliloti
GTP Phosphohydrolases
Chemical activation
Operon
adenylylsulfate kinase
Genes
Gene encoding
Guanine Nucleotides
Initiator Codon
Enzymes
Guanosine Triphosphate
Open Reading Frames
Peptides
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

The DNA sequence of the sulfate activation locus from Escherichia coli K-12. / Leyh, Thomas S.; Vogt, Thomas F.; Suo, Ya.

In: Journal of Biological Chemistry, Vol. 267, No. 15, 25.05.1992, p. 10405-10410.

Research output: Contribution to journalArticle

Leyh, Thomas S. ; Vogt, Thomas F. ; Suo, Ya. / The DNA sequence of the sulfate activation locus from Escherichia coli K-12. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 15. pp. 10405-10410.
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