The distinct temporal origins of olfactory bulb interneuron subtypes

Renata A. Batista-Brito, Jennie Close, Robert Machold, Gord Fishell

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

Olfactory bulb (OB) interneurons are a heterogeneous population produced beginning in embryogenesis and continuing through adulthood. Understanding how this diversity arises will provide insight into how OB microcircuitry is established as well as adult neurogenesis. Particular spatial domains have been shown to contribute specific interneuron subtypes. However, the temporal profile by which OB interneuron subtypes are produced is unknown. Using inducible genetic fate mapping of Dlx1/2 precursors, we analyzed the production of seven OB interneuron subtypes and found that the generation of each subpopulation has a unique temporal signature. Within the glomerular layer, the production of tyrosine hydroxylase-positive interneurons is maximal during early embryogenesis and decreases thereafter. In contrast, the generation of calbindin interneurons is maximal during late embryogenesis and declines postnatally, whereas calretinin (CR) cell production is low during embryogenesis and increases postnatally. Parvalbumin interneurons within the external plexiform layer are produced only perinatally, whereas the generation of 5T4-positive granule cells in the mitral cell layer does not change significantly over time. CR-positive granule cells are not produced at early embryonic time points, but constitute a large percentage of the granule cells born after birth. Blanes cells in contrast are produced in greatest number during embryogenesis. Together we provide the first comprehensive analysis of the temporal generation of OB interneuron subtypes and demonstrate that the timing by which these populations are produced is tightly orchestrated.

Original languageEnglish (US)
Pages (from-to)3966-3975
Number of pages10
JournalJournal of Neuroscience
Volume28
Issue number15
DOIs
StatePublished - Apr 9 2008

Fingerprint

Olfactory Bulb
Interneurons
Embryonic Development
Calbindin 2
Calbindins
Parvalbumins
Neurogenesis
Tyrosine 3-Monooxygenase
Population
Parturition

Keywords

  • Adult stem cells
  • Genetic fate mapping
  • Interneurons
  • Neurogenesis
  • Neuronal birthdate
  • Olfactory bulb

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The distinct temporal origins of olfactory bulb interneuron subtypes. / Batista-Brito, Renata A.; Close, Jennie; Machold, Robert; Fishell, Gord.

In: Journal of Neuroscience, Vol. 28, No. 15, 09.04.2008, p. 3966-3975.

Research output: Contribution to journalArticle

Batista-Brito, Renata A. ; Close, Jennie ; Machold, Robert ; Fishell, Gord. / The distinct temporal origins of olfactory bulb interneuron subtypes. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 15. pp. 3966-3975.
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