The distinct roles of Ras and Rac in PI 3-kinase-dependent protrusion during EGF-stimulated cell migration

Shu Chin Yip, Mirvat El-Sibai, Salvatore J. Coniglio, Ghassan Mouneimne, Robert J. Eddy, Beth E. Drees, Paul O. Neilsen, Sumanta Goswami, Marc Symons, John S. Condeelis, Jonathan M. Backer

Research output: Contribution to journalArticle

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Abstract

Cell migration involves the localized extension of actin-rich protrusions, a process that requires Class I phosphoinositide 3-kinases (PI 3-kinases). Both Rac and Ras have been shown to regulate actin polymerization and activate PI 3-kinase. However, the coordination of Rac, Ras and PI 3-kinase activation during epidermal growth factor (EGF)-stimulated protrusion has not been analyzed. We examined PI 3-kinase-dependent protrusion in MTLn3 rat adenocarcinoma cells. EGF-stimulated phosphatidyl-inositol (3,4,5)-trisphosphate [PtdIns(3,4,5)P3] levels showed a rapid and persistent response, as PI 3-kinase activity remained elevated up to 3 minutes. The activation kinetics of Ras, but not Rac, coincided with those of leading-edge PtdIns(3,4,5)P3 production. Small interfering RNA (siRNA) knockdown of K-Ras but not Rac1 abolished PtdIns(3,4,5)P3 production at the leading edge and inhibited EGF-stimulated protrusion. However, Rac1 knockdown did inhibit cell migration, because of the inhibition of focal adhesion formation in Rac1 siRNA-treated cells. Our data show that in EGF-stimulated MTLn3 carcinoma cells, Ras is required for both PtdIns(3,4,5)P3 production and lamellipod extension, whereas Rac1 is required for formation of adhesive structures. These data suggest an unappreciated role for Ras during protrusion, and a crucial role for Rac in the stabilization of protrusions required for cell motility.

Original languageEnglish (US)
Pages (from-to)3138-3146
Number of pages9
JournalJournal of Cell Science
Volume120
Issue number17
DOIs
StatePublished - Sep 1 2007

Fingerprint

1-Phosphatidylinositol 4-Kinase
Epidermal Growth Factor
Cell Movement
Small Interfering RNA
Actins
Cell Migration Inhibition
Focal Adhesions
Phosphatidylinositols
Polymerization
Adhesives
Adenocarcinoma
Carcinoma
phosphatidylinositol 3,4,5-triphosphate

Keywords

  • Cdc42
  • Lamellipodia
  • Rac
  • Ras
  • Rho

ASJC Scopus subject areas

  • Cell Biology

Cite this

The distinct roles of Ras and Rac in PI 3-kinase-dependent protrusion during EGF-stimulated cell migration. / Yip, Shu Chin; El-Sibai, Mirvat; Coniglio, Salvatore J.; Mouneimne, Ghassan; Eddy, Robert J.; Drees, Beth E.; Neilsen, Paul O.; Goswami, Sumanta; Symons, Marc; Condeelis, John S.; Backer, Jonathan M.

In: Journal of Cell Science, Vol. 120, No. 17, 01.09.2007, p. 3138-3146.

Research output: Contribution to journalArticle

Yip, SC, El-Sibai, M, Coniglio, SJ, Mouneimne, G, Eddy, RJ, Drees, BE, Neilsen, PO, Goswami, S, Symons, M, Condeelis, JS & Backer, JM 2007, 'The distinct roles of Ras and Rac in PI 3-kinase-dependent protrusion during EGF-stimulated cell migration', Journal of Cell Science, vol. 120, no. 17, pp. 3138-3146. https://doi.org/10.1242/jcs.005298
Yip, Shu Chin ; El-Sibai, Mirvat ; Coniglio, Salvatore J. ; Mouneimne, Ghassan ; Eddy, Robert J. ; Drees, Beth E. ; Neilsen, Paul O. ; Goswami, Sumanta ; Symons, Marc ; Condeelis, John S. ; Backer, Jonathan M. / The distinct roles of Ras and Rac in PI 3-kinase-dependent protrusion during EGF-stimulated cell migration. In: Journal of Cell Science. 2007 ; Vol. 120, No. 17. pp. 3138-3146.
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AU - Mouneimne, Ghassan

AU - Eddy, Robert J.

AU - Drees, Beth E.

AU - Neilsen, Paul O.

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AU - Condeelis, John S.

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