TY - JOUR
T1 - The cytoplasmic thioredoxin system in Caenorhabditis elegans affords protection from methylmercury in an age-specific manner
AU - Ruszkiewicz, Joanna A.
AU - Teixeira de Macedo, Gabriel
AU - Miranda-Vizuete, Antonio
AU - Teixeira da Rocha, João B.
AU - Bowman, Aaron B.
AU - Bornhorst, Julia
AU - Schwerdtle, Tanja
AU - Aschner, Michael
N1 - Funding Information:
This work has beensupported by the National Institutes of Health [NIEHS R01ES07331 to MA and ABB]. We thank the German Research Foundation (DFG) for the financial support of BO 4103/2-1 as well as the DFG Research Unit TraceAge ( FOR 2558 ). C. elegans strains were provided by the Caenorhabditis Genetics Center (CGC, University of Minnesota, USA), which is funded by NIH Office of Research Infrastructure Programs [ P40 OD010440 ]. We would like to acknowledge Hillary Guzik for expert assistance in microscopy. The imaging was conducted in the Analytical Imaging Facility, which is funded by the NCI Cancer Grant P30CA013330.
Funding Information:
This work has beensupported by the National Institutes of Health [NIEHS R01ES07331 to MA and ABB]. We thank the German Research Foundation (DFG) for the financial support of BO 4103/2-1 as well as the DFG Research Unit TraceAge (FOR 2558). C. elegans strains were provided by the Caenorhabditis Genetics Center (CGC, University of Minnesota, USA), which is funded by NIH Office of Research Infrastructure Programs [P40 OD010440]. We would like to acknowledge Hillary Guzik for expert assistance in microscopy. The imaging was conducted in the Analytical Imaging Facility, which is funded by the NCI Cancer Grant P30CA013330.
PY - 2018/9
Y1 - 2018/9
N2 - Methylmercury (MeHg) is an environmental pollutant linked to many neurological defects, especially in developing individuals. The thioredoxin (TRX) system is a key redox regulator affected by MeHg toxicity, however the mechanisms and consequences of MeHg-induced dysfunction are not completely understood. This study evaluated the role of the TRX system in C. elegans susceptibility to MeHg during development. Worms lacking or overexpressing proteins from the TRX family were exposed to MeHg for 1 h at different developmental stage: L1, L4 and adult. Worms without cytoplasmic thioredoxin system exhibited age-specific susceptibility to MeHg when compared to wild-type (wt). This susceptibility corresponded partially to decreased total glutathione (GSH) levels and enhanced degeneration of dopaminergic neurons. In contrast, the overexpression of the cytoplasmic system TRX-1/TRXR-1 did not provide substantial protection against MeHg. Moreover, transgenic worms exhibited decreased protein expression for cytoplasmic thioredoxin reductase (TRXR-1). Both mitochondrial thioredoxin system TRX-2/TRXR-2, as well as other thioredoxin-like proteins: TRX-3, TRX-4, TRX-5 did not show significant role in C. elegans resistance to MeHg. Based on the current findings, the cytoplasmic thioredoxin system TRX-1/TRXR-1 emerges as an important age-sensitive protectant against MeHg toxicity in C. elegans.
AB - Methylmercury (MeHg) is an environmental pollutant linked to many neurological defects, especially in developing individuals. The thioredoxin (TRX) system is a key redox regulator affected by MeHg toxicity, however the mechanisms and consequences of MeHg-induced dysfunction are not completely understood. This study evaluated the role of the TRX system in C. elegans susceptibility to MeHg during development. Worms lacking or overexpressing proteins from the TRX family were exposed to MeHg for 1 h at different developmental stage: L1, L4 and adult. Worms without cytoplasmic thioredoxin system exhibited age-specific susceptibility to MeHg when compared to wild-type (wt). This susceptibility corresponded partially to decreased total glutathione (GSH) levels and enhanced degeneration of dopaminergic neurons. In contrast, the overexpression of the cytoplasmic system TRX-1/TRXR-1 did not provide substantial protection against MeHg. Moreover, transgenic worms exhibited decreased protein expression for cytoplasmic thioredoxin reductase (TRXR-1). Both mitochondrial thioredoxin system TRX-2/TRXR-2, as well as other thioredoxin-like proteins: TRX-3, TRX-4, TRX-5 did not show significant role in C. elegans resistance to MeHg. Based on the current findings, the cytoplasmic thioredoxin system TRX-1/TRXR-1 emerges as an important age-sensitive protectant against MeHg toxicity in C. elegans.
KW - Age
KW - C. elegans
KW - Development
KW - Methylmercury
KW - Thioredoxin
KW - Thioredoxin reductase
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U2 - 10.1016/j.neuro.2018.08.007
DO - 10.1016/j.neuro.2018.08.007
M3 - Article
C2 - 30138651
AN - SCOPUS:85052314481
VL - 68
SP - 189
EP - 202
JO - NeuroToxicology
JF - NeuroToxicology
SN - 0161-813X
ER -