The cytoplasmic tail of herpes simplex virus envelope glycoprotein D binds to the tegument protein VP22 and to capsids

Jung Hee I Chi, Carol A. Harley, Aparna Mukhopadhyay, Duncan W. Wilson

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Herpes simplex virus (HSV) capsids assemble, mature and package their viral genome in the nucleoplasm. They then exit the nucleus into the cytoplasm, where they acquire their final tegument and envelope. The molecular mechanism of cytoplasmic envelopment is unclear, but evidence suggests that the viral glycoprotein tails play an important role in the recruitment of tegument and capsids at the final envelopment site. However, due to redundancy in protein-protein interactions among the viral glycoproteins, genetic analysis of the role of individual glycoproteins in assembly has been difficult. To overcome this problem, a glutathione S-transferase fusion protein-binding assay was used in this study to test the interaction between the cytoplasmic tail of one specific viral glycoprotein, gD, and tegument proteins. The study demonstrated that the 38 kDa tegument protein VP22 bound specifically to the gD tail. This association was dependent on arginine and lysine residues at positions 5 and 6 in the gD tail. In addition, HSV-1 capsids bound the gD tail and exhibited a similar sequence dependence. It is concluded that VP22 may serve as a linker protein, mediating the interaction of the HSV capsid with gD.

Original languageEnglish (US)
Pages (from-to)253-261
Number of pages9
JournalJournal of General Virology
Volume86
Issue number2
DOIs
StatePublished - Feb 2005

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Capsid
Simplexvirus
Glycoproteins
Proteins
Viral Genome
Human Herpesvirus 1
Glutathione Transferase
Protein Binding
Lysine
Arginine
Cytoplasm

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

The cytoplasmic tail of herpes simplex virus envelope glycoprotein D binds to the tegument protein VP22 and to capsids. / Chi, Jung Hee I; Harley, Carol A.; Mukhopadhyay, Aparna; Wilson, Duncan W.

In: Journal of General Virology, Vol. 86, No. 2, 02.2005, p. 253-261.

Research output: Contribution to journalArticle

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