The CSF-1 receptor ligands IL-34 and CSF-1 exhibit distinct developmental brain expression patterns and regulate neural progenitor cell maintenance and maturation

Sayan Nandi, Solen Gokhan, Xu Ming Dai, Suwen Wei, Grigori Enikolopov, Haishan Lin, Mark F. Mehler, E. Richard Stanley

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

The CSF-1 receptor (CSF-1R) regulates CNS microglial development. However, the localization and developmental roles of this receptor and its ligands, IL-34 and CSF-1, in the brain are poorly understood. Here we show that compared to wild type mice, CSF-1R-deficient (. Csf1. r-/-) mice have smaller brains of greater mass. They further exhibit an expansion of lateral ventricle size, an atrophy of the olfactory bulb and a failure of midline crossing of callosal axons. In brain, IL-34 exhibited a broader regional expression than CSF-1, mostly without overlap. Expression of IL-34, CSF-1 and the CSF-1R were maximal during early postnatal development. However, in contrast to the expression of its ligands, CSF-1R expression was very low in adult brain. Postnatal neocortical expression showed that CSF-1 was expressed in layer VI, whereas IL-34 was expressed in the meninges and layers II-V. The broader expression of IL-34 is consistent with its previously implicated role in microglial development. The differential expression of CSF-1R ligands, with respect to CSF-1R expression, could reflect their CSF-1R-independent signaling. . Csf1. r-/. - mice displayed increased proliferation and apoptosis of neocortical progenitors and reduced differentiation of specific excitatory neuronal subtypes. Indeed, addition of CSF-1 or IL-34 to microglia-free, CSF-1R-expressing dorsal forebrain clonal cultures, suppressed progenitor self-renewal and enhanced neuronal differentiation. Consistent with a neural developmental role for the CSF-1R, ablation of the . Csf1. r gene in Nestin-positive neural progenitors led to a smaller brain size, an expanded neural progenitor pool and elevated cellular apoptosis in cortical forebrain. Thus our results also indicate novel roles for the CSF-1R in the regulation of corticogenesis.

Original languageEnglish (US)
Pages (from-to)100-113
Number of pages14
JournalDevelopmental Biology
Volume367
Issue number2
DOIs
StatePublished - Jul 15 2012

Fingerprint

Macrophage Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor
Stem Cells
Maintenance
Ligands
Brain
Prosencephalon
Apoptosis
Nestin
Meninges
Corpus Callosum
Lateral Ventricles
Olfactory Bulb
Microglia
Atrophy
Axons

Keywords

  • Cerebral cortex
  • CSF-1
  • IL-34
  • Nestin
  • Neural stem cells
  • Neurogenesis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

The CSF-1 receptor ligands IL-34 and CSF-1 exhibit distinct developmental brain expression patterns and regulate neural progenitor cell maintenance and maturation. / Nandi, Sayan; Gokhan, Solen; Dai, Xu Ming; Wei, Suwen; Enikolopov, Grigori; Lin, Haishan; Mehler, Mark F.; Richard Stanley, E.

In: Developmental Biology, Vol. 367, No. 2, 15.07.2012, p. 100-113.

Research output: Contribution to journalArticle

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AU - Enikolopov, Grigori

AU - Lin, Haishan

AU - Mehler, Mark F.

AU - Richard Stanley, E.

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