TY - JOUR
T1 - The corrected nucleotide sequences of the TaqI restriction and modification enzymes reveal a thirteen-codon overlap
AU - Barany, Francis
AU - Slatko, Barton
AU - Danzitz, Michael
AU - Cowburn, David
AU - Schildkraut, Ira
AU - Wilson, Geoffrey G.
N1 - Funding Information:
We thank Antic Keller for expert technical assistance. We thank Hamilton O. Smith, Don Comb, Anneel Aggar-wal, Richard Gumport, Tom Gingeras, and Marc Kahn for discussions. We thank the Rockefeller University sequencing facility for aa analysis. M.D. is a Norman and Rosita Winston Foundation fellow in Biomedical Research. \]'his work was supported by a grant from the National Institutes of Health (GM-41337-03) to F.B.
PY - 1992/3/1
Y1 - 1992/3/1
N2 - The nucleotide sequence of the genes encoding methyltransferase TaqI (M·TaqI) and restriction endonuclease TaqI (R·TaqI) with the recognition sequence, TCGA, were analyzed in clones isolated from independent libraries. The genes, originally reported as 363 and 236 codons long [Slatko et al., Nucleic Acids Res. 15 (1987)9781-9796] were redetermined as 421 and 263 codons long, respectively. The C terminus of the taqIM gene overlaps the N terminus of the taqIR gene by 13 codons, as observed with the isoschizomeric TthHB8I restriction-modification system [Barany et al., Gene 112 (1992) 13-20]. Removal of the overlapping codons did not interfere with in vivo M·TaqI activity. We postulate the overlap plays a role in regulating taqIR expression.
AB - The nucleotide sequence of the genes encoding methyltransferase TaqI (M·TaqI) and restriction endonuclease TaqI (R·TaqI) with the recognition sequence, TCGA, were analyzed in clones isolated from independent libraries. The genes, originally reported as 363 and 236 codons long [Slatko et al., Nucleic Acids Res. 15 (1987)9781-9796] were redetermined as 421 and 263 codons long, respectively. The C terminus of the taqIM gene overlaps the N terminus of the taqIR gene by 13 codons, as observed with the isoschizomeric TthHB8I restriction-modification system [Barany et al., Gene 112 (1992) 13-20]. Removal of the overlapping codons did not interfere with in vivo M·TaqI activity. We postulate the overlap plays a role in regulating taqIR expression.
KW - Evolution of restriction-modification systems
KW - RNA hairpin structure
KW - overlapping genes
KW - protein-DNA interactions
KW - recombinant DNA
UR - http://www.scopus.com/inward/record.url?scp=0026555919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026555919&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(92)90307-B
DO - 10.1016/0378-1119(92)90307-B
M3 - Article
C2 - 1551602
AN - SCOPUS:0026555919
SN - 0378-1119
VL - 112
SP - 91
EP - 95
JO - Gene
JF - Gene
IS - 1
ER -