The concordance of HPV DNA detection by Hybrid Capture 2 and careHPV on clinician- and self-collected specimens

Wen Chen, Jose Jeronimo, Fang Hui Zhao, You Lin Qiao, Melissa Valdez, Xun Zhang, Le Ni Kang, Pooja Bansil, Proma Paul, Ping Bai, Roger Peck, Jing Li, Feng Chen, Mark H. Stoler, Philip E. Castle

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Abstract

Background: careHPV is a new, lower-cost DNA test for human papillomavirus (HPV). There are limited analytic comparisons of careHPV against a referent HPV DNA test like Hybrid Capture 2 (HC2). Objective: To assess the test agreement between careHPV and HC2 on self- and clinician-collected specimens. Study design: In a population of 7541 women living in rural China, women provided a self-collected (sc) and two clinician-collected (cc) specimens and underwent visual inspection after acetic acid (VIA). The sc specimen and one cc specimen were tested by careHPV and HC2; a random subset of specimens was tested for HPV genotypes. Results: The percent positive on cc specimens and sc specimens was 14.69% and 14.97% for careHPV, respectively, and 15.05% and 18.53% for HC2, respectively; HC2 testing of sc specimens was more likely to test positive than other combinations of tests and specimens (p<0.0001 for all comparisons). The agreement between different tests on the same specimens (kappa=0.787 and 0.691 for cc and sc specimens, respectively) was better than the same test on different specimens (kappa=0.653 and 0.649 for HC2 and careHPV, respectively). Disagreement between the same test on different specimens increased with increasing participant age (ptrend=0.0001 for HC2 and 0.002 for careHPV). HC2-positive/careHPV-negative specimens were more likely to test positive for non-carcinogenic HPV genotype than test HPV negative whereas the converse was true for HC2-negative/careHPV-positive specimens. Discussion: The agreement for HPV DNA detection between careHPV and HC2 was good to very good.

Original languageEnglish (US)
Pages (from-to)553-557
Number of pages5
JournalJournal of Clinical Virology
Volume61
Issue number4
DOIs
StatePublished - Jan 1 2014

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Keywords

  • Cervical cancer
  • Cervical intraepithelial neoplasia (CIN)
  • Human papillomavirus (HPV)
  • Visual inspection after acetic acid (VIA)

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Chen, W., Jeronimo, J., Zhao, F. H., Qiao, Y. L., Valdez, M., Zhang, X., Kang, L. N., Bansil, P., Paul, P., Bai, P., Peck, R., Li, J., Chen, F., Stoler, M. H., & Castle, P. E. (2014). The concordance of HPV DNA detection by Hybrid Capture 2 and careHPV on clinician- and self-collected specimens. Journal of Clinical Virology, 61(4), 553-557. https://doi.org/10.1016/j.jcv.2014.09.018