Abstract
Background: careHPV is a new, lower-cost DNA test for human papillomavirus (HPV). There are limited analytic comparisons of careHPV against a referent HPV DNA test like Hybrid Capture 2 (HC2). Objective: To assess the test agreement between careHPV and HC2 on self- and clinician-collected specimens. Study design: In a population of 7541 women living in rural China, women provided a self-collected (sc) and two clinician-collected (cc) specimens and underwent visual inspection after acetic acid (VIA). The sc specimen and one cc specimen were tested by careHPV and HC2; a random subset of specimens was tested for HPV genotypes. Results: The percent positive on cc specimens and sc specimens was 14.69% and 14.97% for careHPV, respectively, and 15.05% and 18.53% for HC2, respectively; HC2 testing of sc specimens was more likely to test positive than other combinations of tests and specimens (p<0.0001 for all comparisons). The agreement between different tests on the same specimens (kappa=0.787 and 0.691 for cc and sc specimens, respectively) was better than the same test on different specimens (kappa=0.653 and 0.649 for HC2 and careHPV, respectively). Disagreement between the same test on different specimens increased with increasing participant age (ptrend=0.0001 for HC2 and 0.002 for careHPV). HC2-positive/careHPV-negative specimens were more likely to test positive for non-carcinogenic HPV genotype than test HPV negative whereas the converse was true for HC2-negative/careHPV-positive specimens. Discussion: The agreement for HPV DNA detection between careHPV and HC2 was good to very good.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 553-557 |
| Number of pages | 5 |
| Journal | Journal of Clinical Virology |
| Volume | 61 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2014 |
| Externally published | Yes |
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Keywords
- Cervical cancer
- Cervical intraepithelial neoplasia (CIN)
- Human papillomavirus (HPV)
- Visual inspection after acetic acid (VIA)
ASJC Scopus subject areas
- Virology
- Infectious Diseases
- Medicine(all)
Cite this
The concordance of HPV DNA detection by Hybrid Capture 2 and careHPV on clinician- and self-collected specimens. / Chen, Wen; Jeronimo, Jose; Zhao, Fang Hui; Qiao, You Lin; Valdez, Melissa; Zhang, Xun; Kang, Le Ni; Bansil, Pooja; Paul, Proma; Bai, Ping; Peck, Roger; Li, Jing; Chen, Feng; Stoler, Mark H.; Castle, Philip E.
In: Journal of Clinical Virology, Vol. 61, No. 4, 2014, p. 553-557.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The concordance of HPV DNA detection by Hybrid Capture 2 and careHPV on clinician- and self-collected specimens
AU - Chen, Wen
AU - Jeronimo, Jose
AU - Zhao, Fang Hui
AU - Qiao, You Lin
AU - Valdez, Melissa
AU - Zhang, Xun
AU - Kang, Le Ni
AU - Bansil, Pooja
AU - Paul, Proma
AU - Bai, Ping
AU - Peck, Roger
AU - Li, Jing
AU - Chen, Feng
AU - Stoler, Mark H.
AU - Castle, Philip E.
PY - 2014
Y1 - 2014
N2 - Background: careHPV is a new, lower-cost DNA test for human papillomavirus (HPV). There are limited analytic comparisons of careHPV against a referent HPV DNA test like Hybrid Capture 2 (HC2). Objective: To assess the test agreement between careHPV and HC2 on self- and clinician-collected specimens. Study design: In a population of 7541 women living in rural China, women provided a self-collected (sc) and two clinician-collected (cc) specimens and underwent visual inspection after acetic acid (VIA). The sc specimen and one cc specimen were tested by careHPV and HC2; a random subset of specimens was tested for HPV genotypes. Results: The percent positive on cc specimens and sc specimens was 14.69% and 14.97% for careHPV, respectively, and 15.05% and 18.53% for HC2, respectively; HC2 testing of sc specimens was more likely to test positive than other combinations of tests and specimens (p<0.0001 for all comparisons). The agreement between different tests on the same specimens (kappa=0.787 and 0.691 for cc and sc specimens, respectively) was better than the same test on different specimens (kappa=0.653 and 0.649 for HC2 and careHPV, respectively). Disagreement between the same test on different specimens increased with increasing participant age (ptrend=0.0001 for HC2 and 0.002 for careHPV). HC2-positive/careHPV-negative specimens were more likely to test positive for non-carcinogenic HPV genotype than test HPV negative whereas the converse was true for HC2-negative/careHPV-positive specimens. Discussion: The agreement for HPV DNA detection between careHPV and HC2 was good to very good.
AB - Background: careHPV is a new, lower-cost DNA test for human papillomavirus (HPV). There are limited analytic comparisons of careHPV against a referent HPV DNA test like Hybrid Capture 2 (HC2). Objective: To assess the test agreement between careHPV and HC2 on self- and clinician-collected specimens. Study design: In a population of 7541 women living in rural China, women provided a self-collected (sc) and two clinician-collected (cc) specimens and underwent visual inspection after acetic acid (VIA). The sc specimen and one cc specimen were tested by careHPV and HC2; a random subset of specimens was tested for HPV genotypes. Results: The percent positive on cc specimens and sc specimens was 14.69% and 14.97% for careHPV, respectively, and 15.05% and 18.53% for HC2, respectively; HC2 testing of sc specimens was more likely to test positive than other combinations of tests and specimens (p<0.0001 for all comparisons). The agreement between different tests on the same specimens (kappa=0.787 and 0.691 for cc and sc specimens, respectively) was better than the same test on different specimens (kappa=0.653 and 0.649 for HC2 and careHPV, respectively). Disagreement between the same test on different specimens increased with increasing participant age (ptrend=0.0001 for HC2 and 0.002 for careHPV). HC2-positive/careHPV-negative specimens were more likely to test positive for non-carcinogenic HPV genotype than test HPV negative whereas the converse was true for HC2-negative/careHPV-positive specimens. Discussion: The agreement for HPV DNA detection between careHPV and HC2 was good to very good.
KW - Cervical cancer
KW - Cervical intraepithelial neoplasia (CIN)
KW - Human papillomavirus (HPV)
KW - Visual inspection after acetic acid (VIA)
UR - http://www.scopus.com/inward/record.url?scp=84922744895&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922744895&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2014.09.018
DO - 10.1016/j.jcv.2014.09.018
M3 - Article
C2 - 25453572
AN - SCOPUS:84922744895
VL - 61
SP - 553
EP - 557
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
IS - 4
ER -