TY - JOUR
T1 - The complete amino acid sequence of human brain-derived acidic fibroblast growth factor
AU - Gimenez-Gallego, Guillermo
AU - Conn, Greg
AU - Hatcher, Victor B.
AU - Thomas, Kenneth A.
N1 - Funding Information:
The work was also funded by grants from the U.S. Public Health Service (AG
PY - 1986/7/31
Y1 - 1986/7/31
N2 - Acidic fibroblast growth factor is a potent mitogen for a variety of cells in culture, including vascular endothelial cells, and is angiogenic in vivo. The complete amino acid sequence of human brain-derived acidic fibroblast growth factor has been determined from amino terminal sequence analysis and carboxypeptidase A digestion of the whole protein and sequence analyses of peptides generated by tryptic, Staphylococcus aureus V8 protease and cyanogen bromide cleavages. A potential Asn-Gly-Ser glycosylation sequence is present in the human protein. The complete amino acid sequence is compared to that of the equivalent protein purified from bovine brain.
AB - Acidic fibroblast growth factor is a potent mitogen for a variety of cells in culture, including vascular endothelial cells, and is angiogenic in vivo. The complete amino acid sequence of human brain-derived acidic fibroblast growth factor has been determined from amino terminal sequence analysis and carboxypeptidase A digestion of the whole protein and sequence analyses of peptides generated by tryptic, Staphylococcus aureus V8 protease and cyanogen bromide cleavages. A potential Asn-Gly-Ser glycosylation sequence is present in the human protein. The complete amino acid sequence is compared to that of the equivalent protein purified from bovine brain.
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U2 - 10.1016/S0006-291X(86)80540-X
DO - 10.1016/S0006-291X(86)80540-X
M3 - Article
C2 - 3527167
AN - SCOPUS:0022512063
SN - 0006-291X
VL - 138
SP - 611
EP - 617
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -