The complete amino acid sequence of human brain-derived acidic fibroblast growth factor

Guillermo Gimenez-Gallego, Greg Conn, Victor Bernard Hatcher, Kenneth A. Thomas

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Acidic fibroblast growth factor is a potent mitogen for a variety of cells in culture, including vascular endothelial cells, and is angiogenic in vivo. The complete amino acid sequence of human brain-derived acidic fibroblast growth factor has been determined from amino terminal sequence analysis and carboxypeptidase A digestion of the whole protein and sequence analyses of peptides generated by tryptic, Staphylococcus aureus V8 protease and cyanogen bromide cleavages. A potential Asn-Gly-Ser glycosylation sequence is present in the human protein. The complete amino acid sequence is compared to that of the equivalent protein purified from bovine brain.

Original languageEnglish (US)
Pages (from-to)611-617
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number2
DOIs
StatePublished - Jul 31 1986

Fingerprint

Fibroblast Growth Factor 1
Amino Acid Sequence
Brain
Carboxypeptidases A
Amino Acids
Cyanogen Bromide
Protein Sequence Analysis
Glycosylation
Mitogens
Sequence Analysis
Staphylococcus aureus
Digestion
Proteins
Endothelial Cells
Cell Culture Techniques
Endothelial cells
Cell culture
Peptides
glutamyl endopeptidase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The complete amino acid sequence of human brain-derived acidic fibroblast growth factor. / Gimenez-Gallego, Guillermo; Conn, Greg; Hatcher, Victor Bernard; Thomas, Kenneth A.

In: Biochemical and Biophysical Research Communications, Vol. 138, No. 2, 31.07.1986, p. 611-617.

Research output: Contribution to journalArticle

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