The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization

Stefano Morettini, Martin Tribus, Anette Zeilner, Johanna Sebald, Beatriz Campo-Fernandez, Gabriele Scheran, Hildegard Wörle, Valerie Podhraski, Dmitry Fyodorov, Alexandra Lusser

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The molecular motor protein CHD1 has been implicated in the regulation of transcription and in the transcription-independent genome-wide incorporation of H3.3 into paternal chromatin in Drosophila melanogaster. A key feature of CHD1 is the presence of two chromodomains, which can bind to histone H3 methylated at lysine 4 and thus might serve to recruit and/or maintain CHD1 at the chromatin. Here, we describe genetic and biochemical approaches to the study of the Drosophila CHD1 chromodomains. We found that overall localization of CHD1 on polytene chromosomes does not appreciably change in chromodomain-mutant flies. In contrast, the chromodomains are important for transcription-independent activities of CHD1 during early embryonic development as well as for transcriptional regulation of several heat shock genes. However, neither CHD1 nor its chromodomains are needed for RNA polymerase II localization and H3K4 methylation but loss of CHD1 decreases transcription-induced histone eviction at the Hsp70 gene in vivo. Chromodomain mutations negatively affect the chromatin assembly activities of CHD1 in vitro, and they appear to be involved in linking the ATP-dependent motor to the chromatin assembly function of CHD1.

Original languageEnglish (US)
Pages (from-to)3103-3115
Number of pages13
JournalNucleic Acids Research
Volume39
Issue number8
DOIs
StatePublished - Apr 2011

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Chromatin Assembly and Disassembly
Histones
Chromatin
Molecular Motor Proteins
Polytene Chromosomes
RNA Polymerase II
Drosophila melanogaster
Diptera
Methylation
Genes
Lysine
Drosophila
Embryonic Development
Molecular Biology
Shock
Hot Temperature
Adenosine Triphosphate
Genome
Mutation
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics

Cite this

Morettini, S., Tribus, M., Zeilner, A., Sebald, J., Campo-Fernandez, B., Scheran, G., ... Lusser, A. (2011). The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization. Nucleic Acids Research, 39(8), 3103-3115. https://doi.org/10.1093/nar/gkq1298

The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization. / Morettini, Stefano; Tribus, Martin; Zeilner, Anette; Sebald, Johanna; Campo-Fernandez, Beatriz; Scheran, Gabriele; Wörle, Hildegard; Podhraski, Valerie; Fyodorov, Dmitry; Lusser, Alexandra.

In: Nucleic Acids Research, Vol. 39, No. 8, 04.2011, p. 3103-3115.

Research output: Contribution to journalArticle

Morettini, S, Tribus, M, Zeilner, A, Sebald, J, Campo-Fernandez, B, Scheran, G, Wörle, H, Podhraski, V, Fyodorov, D & Lusser, A 2011, 'The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization', Nucleic Acids Research, vol. 39, no. 8, pp. 3103-3115. https://doi.org/10.1093/nar/gkq1298
Morettini S, Tribus M, Zeilner A, Sebald J, Campo-Fernandez B, Scheran G et al. The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization. Nucleic Acids Research. 2011 Apr;39(8):3103-3115. https://doi.org/10.1093/nar/gkq1298
Morettini, Stefano ; Tribus, Martin ; Zeilner, Anette ; Sebald, Johanna ; Campo-Fernandez, Beatriz ; Scheran, Gabriele ; Wörle, Hildegard ; Podhraski, Valerie ; Fyodorov, Dmitry ; Lusser, Alexandra. / The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization. In: Nucleic Acids Research. 2011 ; Vol. 39, No. 8. pp. 3103-3115.
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