The Cerebral Cavernous Malformation Pathway Controls Cardiac Development via Regulation of Endocardial MEKK3 Signaling and KLF Expression

Zinan Zhou, David R. Rawnsley, Lauren M. Goddard, Wei Pan, Xing Jun Cao, Zoltan Jakus, Hui Zheng, Jisheng Yang, J. Simon C. Arthur, Kevin J. Whitehead, Dean Li, Bin Zhou, Benjamin A. Garcia, Xiangjian Zheng, Mark L. Kahn

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

The cerebral cavernous malformation (CCM) pathway is required in endothelial cells for normal cardiovascular development and to prevent postnatal vascular malformations, but its molecular effectors are not well defined. Here we show that loss of CCM signaling in endocardial cells results in mid-gestation heart failure associated with premature degradation of cardiac jelly. CCM deficiency dramatically alters endocardial and endothelial gene expression, including increased expression of the Klf2 and Klf4 transcription factors and the Adamts4 and Adamts5 proteases that degrade cardiac jelly. These changes in gene expression result from increased activity of MEKK3, a mitogen-activated protein kinase that binds CCM2 in endothelial cells. MEKK3 is both necessary and sufficient for expression of these genes, and partial loss of MEKK3 rescues cardiac defects in CCM-deficient embryos. These findings reveal a molecular mechanism by which CCM signaling controls endothelial gene expression during cardiovascular development that may also underlie CCM formation.

Original languageEnglish (US)
Pages (from-to)168-180
Number of pages13
JournalDevelopmental cell
Volume32
Issue number2
DOIs
StatePublished - Jan 26 2015

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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    Zhou, Z., Rawnsley, D. R., Goddard, L. M., Pan, W., Cao, X. J., Jakus, Z., Zheng, H., Yang, J., Arthur, J. S. C., Whitehead, K. J., Li, D., Zhou, B., Garcia, B. A., Zheng, X., & Kahn, M. L. (2015). The Cerebral Cavernous Malformation Pathway Controls Cardiac Development via Regulation of Endocardial MEKK3 Signaling and KLF Expression. Developmental cell, 32(2), 168-180. https://doi.org/10.1016/j.devcel.2014.12.009