The Cdk-Activating Kinase Cak1p Promotes Meiotic S Phase through Ime2p

Karen Schindler, Kirsten R. Benjamin, Allison Martin, Andrew Boglioli, Ira Herskowitz, Edward Winter

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

CAK1 encodes an essential protein kinase in Saccharomyces cerevisiae that is required for activation of the Cdc28p Cdk. CAK1 also has several CDC28-independent functions that are unique to meiosis. The earliest of these functions is to induce S phase, which is regulated differently in meiosis than in mitosis. In mitosis, Cdc28p controls its own S-phase-promoting activity by signaling the destruction of its inhibitor, Sic1p. In meiosis, Sic1p destruction is signaled by the meiosis-specific Ime2p protein kinase. Our data show that Cak1p is required to activate Ime2p through a mechanism that requires threonine 242 and tyrosine 244 in Ime2p's activation loop. This activation promotes autophosphorylation and accumulation of multiply phosphorylated forms of Ime2p during meiotic development. Consistent with Cak1p's role in activating Ime2p, cells lacking Cak1p are deficient in degrading Sic1p. Deletion of SIC1 or overexpression of IME2 can partially suppress the S-phase defect in cak1 mutant cells, suggesting that Ime2p is a key target of Cak1p regulation. These data show that Cak1p is required for the destruction of Sic1p in meiosis, as in mitosis, but in meiosis, it functions through a sporulation-specific kinase.

Original languageEnglish (US)
Pages (from-to)8718-8728
Number of pages11
JournalMolecular and Cellular Biology
Volume23
Issue number23
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

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Meiosis
S Phase
Mitosis
Protein Kinases
Threonine
Saccharomyces cerevisiae
Tyrosine
cyclin-dependent kinase-activating kinase
Phosphotransferases

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The Cdk-Activating Kinase Cak1p Promotes Meiotic S Phase through Ime2p. / Schindler, Karen; Benjamin, Kirsten R.; Martin, Allison; Boglioli, Andrew; Herskowitz, Ira; Winter, Edward.

In: Molecular and Cellular Biology, Vol. 23, No. 23, 01.12.2003, p. 8718-8728.

Research output: Contribution to journalArticle

Schindler, K, Benjamin, KR, Martin, A, Boglioli, A, Herskowitz, I & Winter, E 2003, 'The Cdk-Activating Kinase Cak1p Promotes Meiotic S Phase through Ime2p', Molecular and Cellular Biology, vol. 23, no. 23, pp. 8718-8728. https://doi.org/10.1128/MCB.23.23.8718-8728.2003
Schindler, Karen ; Benjamin, Kirsten R. ; Martin, Allison ; Boglioli, Andrew ; Herskowitz, Ira ; Winter, Edward. / The Cdk-Activating Kinase Cak1p Promotes Meiotic S Phase through Ime2p. In: Molecular and Cellular Biology. 2003 ; Vol. 23, No. 23. pp. 8718-8728.
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