The C. elegans POU-domain transcription factor UNC-86 regulates the tph-1 tryptophan hydroxylase gene and neurite outgrowth in specific serotonergic neurons

Ji Ying Sze, Shenyuan Zhang, Jie Li, Gary Ruvkun

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A fundamental question in developmental neurobiology is how a common neurotransmitter is specified in different neuronal types?. We describe cell-specific regulation of the serotonergic phenotype by the C. elegans POU-transcription factor UNC-86. We show that unc-86 regulates particular aspects of the terminal neuronal identity in four classes of serotonergic neurons, but that the development of the ADF serotonergic neurons is regulated by an UNC-86-independent program. In the NSM neurons, the role of unc-86 is confined in late differentiation; the neurons are generated but do not express genes necessary for serotonergic neurotransmission. unc-86-null mutations affect the expression in NSM of tph-1, which encodes the serotonin synthetic enzyme tryptophan hydroxylase, and cat-1, which encodes a vesicular transporter that loads serotonin into synaptic vesicles, suggesting that unc-86 coordinately regulates serotonin synthesis and packaging. However, unc-86-null mutations do not impair the ability of NSM to reuptake serotonin released from the ADF serotonergic chemosensory neurons and this serotonin reuptake is sensitive to the serotonin reuptake block drugs imipramine and fluoxetine, demonstrating that serotonin synthesis and reuptake is regulated by distinct factors. The NSM neurons in unc-86-null mutants also display abnormal neurite outgrowth, suggesting a role of unc-86 in regulating this process as well.

Original languageEnglish (US)
Pages (from-to)3901-3911
Number of pages11
JournalDevelopment
Volume129
Issue number16
StatePublished - Aug 2002
Externally publishedYes

Fingerprint

POU Domain Factors
Tryptophan Hydroxylase
Serotonergic Neurons
Serotonin
Genes
Neurons
Serotonin Plasma Membrane Transport Proteins
Mutation
Neurobiology
Synaptic Vesicles
Imipramine
Fluoxetine
Product Packaging
Synaptic Transmission
Neurotransmitter Agents
Neuronal Outgrowth
Cats
Phenotype
Enzymes
Pharmaceutical Preparations

Keywords

  • Axon guidance
  • C. elegans
  • Neuron identity
  • Neurotransmitter
  • POU domain
  • Serotonin
  • Serotonin reuptake inhibitors
  • Transcription

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

The C. elegans POU-domain transcription factor UNC-86 regulates the tph-1 tryptophan hydroxylase gene and neurite outgrowth in specific serotonergic neurons. / Sze, Ji Ying; Zhang, Shenyuan; Li, Jie; Ruvkun, Gary.

In: Development, Vol. 129, No. 16, 08.2002, p. 3901-3911.

Research output: Contribution to journalArticle

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