The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity

Julien Vandamme, Gaëlle Lettier, Simone Sidoli, Elia Di Schiavi, Ole Nørregaard Jensen, Anna Elisabetta Salcini

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Epigenetic modifications influence gene expression and provide a unique mechanism for fine-tuning cellular differentiation and development in multicellular organisms. Here we report on the biological functions of UTX-1, the Caenorhabditis elegans homologue of mammalian UTX, a histone demethylase specific for H3K27me2/3. We demonstrate that utx-1 is an essential gene that is required for correct embryonic and postembryonic development. Consistent with its homology to UTX, UTX-1 regulates global levels of H3K27me2/3 in C. elegans. Surprisingly, we found that the catalytic activity is not required for the developmental function of this protein. Biochemical analysis identified UTX-1 as a component of a complex that includes SET-16(MLL), and genetic analysis indicates that the defects associated with loss of UTX-1 are likely mediated by compromised SET-16/UTX-1 complex activity. Taken together, these results demonstrate that UTX-1 is required for many aspects of nematode development; but, unexpectedly, this function is independent of its enzymatic activity.

Original languageEnglish (US)
Article numbere1002647
JournalPLoS genetics
Volume8
Issue number5
DOIs
StatePublished - May 1 2012
Externally publishedYes

Fingerprint

Caenorhabditis elegans
genetic analysis
homology
gene expression
defect
nematode
Histone Demethylases
protein
gene
Essential Genes
catalytic activity
Epigenomics
histones
epigenetics
Embryonic Development
genetic techniques and protocols
Nematoda
Gene Expression
organisms
Proteins

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity. / Vandamme, Julien; Lettier, Gaëlle; Sidoli, Simone; Di Schiavi, Elia; Nørregaard Jensen, Ole; Salcini, Anna Elisabetta.

In: PLoS genetics, Vol. 8, No. 5, e1002647, 01.05.2012.

Research output: Contribution to journalArticle

Vandamme, Julien ; Lettier, Gaëlle ; Sidoli, Simone ; Di Schiavi, Elia ; Nørregaard Jensen, Ole ; Salcini, Anna Elisabetta. / The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity. In: PLoS genetics. 2012 ; Vol. 8, No. 5.
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