The branch site adenosine is recognized differently for the two steps of pre-mRNA splicing.

C. C. Query, S. A. Strobel, P. A. Sharp

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The functional groups responsible for branch site identity in the two steps of pre-mRNA splicing as well as for spliceosome assembly were tested by incorporation of site-specific modifications at the branch site of a pre-mRNA. These results show that recognition of the adenosine occurs early in complex formation and that the branch site adenosine is recognized differently before the first step and for the second step. Further, direct UV cross-linking with these modified RNAs was used to identify a factor whose interaction was dependent upon the identity of the branch site nucleotide.

Original languageEnglish (US)
Pages (from-to)224-225
Number of pages2
JournalNucleic acids symposium series
Issue number33
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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