@article{42657641a7554f3d805cccbc15a5d4e1,
title = "The Atg6/Vps30/Beclin 1 ortholog BEC-1 mediates endocytic retrograde transport in addition to autophagy in C. elegans",
abstract = "Autophagy and endocytosis are dynamic and tightly regulated processes that contribute to many fundamental aspects of biology including survival, longevity and development. However, the molecular links between autophagy and endocytosis are not well understood. Here, we report that BEC -1, the C. elegans ortholog of Atg6/Vps30/Beclin 1, a key regulator of the autophagic machinery, also contributes to endosome function. In particular we identified a defect in retrograde transport from endosomes to the Golgi in bec-1 mutants. MIG-14/Wntless is normally recycled from endosomes to the Golgi through the action of the retromer complex and its associated factor RME-8. Lack of retromer or RME-8 activity results in the aberrant transport of MIG-14/Wntless to the lysosome where it is degraded. Similarly, we found that lack of bec-1 also results in mislocalization and degradation of MIG-14::GFP, reduced levels of RME-8 on endosomal membranes, and the accumulation of morphologically abnormal endosomes. A similar phenotype was observed in animals treated with dsRNA against vps-34. We further identified a requirement for BEC-1 in the clearance of apoptotic corpses in the hermaphrodite gonad, suggesting a role for BEC-1 in phagosome maturation, a process that appears to depend upon retrograde transport. In addition, autophagy genes may also be required for cell corpse clearance, as we found that RNAi against atg-18 or unc-51 also results in a lack of cell corpse clearance.",
keywords = "Autophagy, C. elegans, Endocytosis, Lysosomes",
author = "Alexander Ruck and John Attonito and Garces, {Kelly T.} and Lizbeth N{\'u}nez and Palmisano, {Nicholas J.} and Zahava Rubel and Zhiyong Bai and Nguyen, {Ken C.Q.} and Lei Sun and Grant, {Barth D.} and Hall, {David H.} and Alicia Mel{\'e}ndez",
note = "Funding Information: {\"U} -BOEFT#JPTDJFODF were then infiltrated with epoxy resin and heat cured. These plas-Drs. Cathy Savage-Dunn, Hannes B{\"u}low and Iva Greenwald for tic blocks were thin sectioned, post-stained with uranyl acetate critical reading of this manuscript and for helpful discussions. and lead citrate, and then examined by electron microscopy. This work was supported in part by grants from the NIH/NIA Funding Information: cell corpses was measured uQuantitation of cell co%sing trpses.Phe CEDThe incO-1::Grease iPFP man the nUrker. umbEAdulter ofJTand A(AG0U.M248S., an82-Jd f04Crom thS1) to DVe NSr. MUF, RonFesearch Initiation Grant 0818802 ica Driscoll (Rutgers University) hermaphrodites at 36 h post-larval L4 stage animals were scored. to A.M. A.M. is an Ellison Medical Foundation New Scholar Quantification of germ cell corpses in wild-type animals after in Aging (AG-NS-0521-08). D.H.H. is supported by NIH RR treatment with control RNAi, unc-51/ATG1, bec-1, vps-34 and 12596. B.D.G. is supported by NIH Grant GM067237. atg-18 RNAi animals. Data are derived from three experiments observing adults, 36 h post-larval L4 stage. Data were compared by analysis of variance (ANOVA) and five two-sample t-tests between control strain and the five treatments; 30 animals were",
year = "2011",
month = apr,
doi = "10.4161/auto.7.4.14391",
language = "English (US)",
volume = "7",
pages = "386--400",
journal = "Autophagy",
issn = "1554-8627",
publisher = "Landes Bioscience",
number = "4",
}