The association of tubulin carboxypeptidase activity with microtubules in brain extracts is modulated by phosphorylation/dephosphorylation processes

Juan J. Sironi, Héctor S. Barra, Carlos A. Arce

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tubulin carboxypeptidase, the enzyme which releases the COOH terminal tyrosine from the α-chain of tubulin, remains associated with microtubules through several cycles of assembly/disassembly. Here, we present evidence indicating that in rat brain extract the carboxypeptidase/microtubules association is regulated by the relative activities of endogenous protein kinase(s) and phosphatase(s) which seem to determine the phosphorylation state of the enzyme (or another entity) and in some way the affinity of the enzyme for microtubules. The presence of 2.5 mM ATP during the in vitro microtubule formation resulted in a low recovery of carboxypeptidase activity in the microtubule fraction. This ATP-induced effect was not due to alteration of the enzyme activity or to inhibition of microtubule assembly but to a decrease of the association of the enzyme with microtubules. We found that the ATP-induced effect was not mediated by modifications on the microtubules but, presumably, on the enzyme molecule. The non-hydrolyzable ATP analogue, AMP-PCP, did not reproduce the effect of ATP. The inclusion of phosphatase inhibitors in the homogenization buffer also led to a decrease in the amount of tubulin carboxypeptidase associated with microtubules. Finally, we found that, in concordance with the mechanism hypothesized, the magnitude of the carboxypeptidase/microtubule association correlated well with the different incubation conditions created to favor maximal, minimal or intermediate protein phosphorylation states.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalMolecular and Cellular Biochemistry
Volume170
Issue number1-2
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Phosphorylation
Microtubules
Brain
Carboxypeptidases
Adenosine Triphosphate
Association reactions
Enzymes
Phosphoric Monoester Hydrolases
Enzyme inhibition
Enzyme activity
Tubulin
Protein Kinases
Tyrosine
Rats
Buffers
tyrosyltubulin carboxypeptidase
Recovery
Molecules
Phosphoprotein Phosphatases
Proteins

Keywords

  • Carboxypeptidase/microtubules association
  • Microtubules
  • Tubulin carboxypeptidase
  • Tyrosination state

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The association of tubulin carboxypeptidase activity with microtubules in brain extracts is modulated by phosphorylation/dephosphorylation processes. / Sironi, Juan J.; Barra, Héctor S.; Arce, Carlos A.

In: Molecular and Cellular Biochemistry, Vol. 170, No. 1-2, 1997, p. 9-16.

Research output: Contribution to journalArticle

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